A simple and robust fluorescent labeling method to quantify trophoblast fusion.

Published

Journal Article

Trophoblast fusion into syncytiotrophoblasts is a specialized yet enigmatic cellular process, which is essential for placental development and function. To facilitate mechanistic understanding of this critical process, here we re-purposed a widely used fluorescent membrane potential dye, Di-8-ANEPPS, to stably label the plasma membrane of live BeWo trophoblast cells. Compared to the methods currently available to quantify trophoblast fusion, our new fluorescent labeling method is simple, economical, robust and versatile, enabling quick and accurate quantification of fusion index in living cells.

Full Text

Duke Authors

Cited Authors

  • Zhang, Y; Yang, H

Published Date

  • February 2019

Published In

Volume / Issue

  • 77 /

Start / End Page

  • 16 - 18

PubMed ID

  • 30827351

Pubmed Central ID

  • 30827351

Electronic International Standard Serial Number (EISSN)

  • 1532-3102

Digital Object Identifier (DOI)

  • 10.1016/j.placenta.2019.01.016

Language

  • eng

Conference Location

  • Netherlands