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Perfluorooctyl bromide (perflubron) attenuates oxidative injury to biological and nonbiological systems.

Publication ,  Journal Article
Rotta, AT; Gunnarsson, B; Fuhrman, BP; Wiryawan, B; Hernan, LJ; Steinhorn, DM
Published in: Pediatric critical care medicine : a journal of the Society of Critical Care Medicine and the World Federation of Pediatric Intensive and Critical Care Societies
April 2003

To examine whether perfluorooctyl bromide (perflubron) is capable of protecting biological and nonbiological systems against oxidative damage through a mechanism independent of its known anti-inflammatory property.A controlled, in vitro laboratory study.Research laboratory of a health sciences university.Rat pulmonary artery endothelial cell cultures (biological system) and linoleic acid in sodium dodecyl sulfate micelles (nonbiological system).Rat pulmonary artery endothelial cells labeled with dichlorofluorescein diacetate and incubated with perflubron or culture media (control) were exposed to H2O2. H2O2-induced fluorescence of dichlorofluorescein diacetate was measured as an index of intracellular oxidative stress. In another experiment, linoleic acid in sodium dodecyl sulfate micelles was exposed to various concentrations of the azo initiator 2,2'-diazo-bis-(2-amidinopropane) dihydrochloride (2, 4, 20, and 50 mM) in the presence or absence of perflubron. Malondialdehyde measurements were obtained as a marker of oxidative damage to linoleic acid.Cell monolayers incubated with perflubron exhibited 66.6% attenuation in intracellular fluorescence compared with controls (p < .05). Linoleic acid in sodium dodecyl sulfate micelles incubated with perflubron and exposed to 2, 4, 20, or 50 mM of 2,2'-diazo-bis-(2-amidinopropane) dihydrochloride showed less evidence of lipid peroxidation as indicated by lower malondialdehyde measurements at 240 mins (10.6%, 16%, 41%, and 14.2%, respectively) compared with controls.Perflubron attenuates oxidative damage to both biological and nonbiological systems. This newly recognized property of perflubron is independent of its anti-inflammatory properties.

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Published In

Pediatric critical care medicine : a journal of the Society of Critical Care Medicine and the World Federation of Pediatric Intensive and Critical Care Societies

DOI

ISSN

1529-7535

Publication Date

April 2003

Volume

4

Issue

2

Start / End Page

233 / 238

Related Subject Headings

  • Rats
  • Pulmonary Artery
  • Pediatrics
  • Oxidative Stress
  • Muscle, Smooth, Vascular
  • Liquid Ventilation
  • Lipid Peroxidation
  • Linoleic Acid
  • Hydrocarbons, Brominated
  • Fluorocarbons
 

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Rotta, A. T., Gunnarsson, B., Fuhrman, B. P., Wiryawan, B., Hernan, L. J., & Steinhorn, D. M. (2003). Perfluorooctyl bromide (perflubron) attenuates oxidative injury to biological and nonbiological systems. Pediatric Critical Care Medicine : A Journal of the Society of Critical Care Medicine and the World Federation of Pediatric Intensive and Critical Care Societies, 4(2), 233–238. https://doi.org/10.1097/01.pcc.0000059729.21375.d0
Rotta, Alexandre T., Björn Gunnarsson, Bradley P. Fuhrman, Budi Wiryawan, Lynn J. Hernan, and David M. Steinhorn. “Perfluorooctyl bromide (perflubron) attenuates oxidative injury to biological and nonbiological systems.Pediatric Critical Care Medicine : A Journal of the Society of Critical Care Medicine and the World Federation of Pediatric Intensive and Critical Care Societies 4, no. 2 (April 2003): 233–38. https://doi.org/10.1097/01.pcc.0000059729.21375.d0.
Rotta AT, Gunnarsson B, Fuhrman BP, Wiryawan B, Hernan LJ, Steinhorn DM. Perfluorooctyl bromide (perflubron) attenuates oxidative injury to biological and nonbiological systems. Pediatric critical care medicine : a journal of the Society of Critical Care Medicine and the World Federation of Pediatric Intensive and Critical Care Societies. 2003 Apr;4(2):233–8.
Rotta, Alexandre T., et al. “Perfluorooctyl bromide (perflubron) attenuates oxidative injury to biological and nonbiological systems.Pediatric Critical Care Medicine : A Journal of the Society of Critical Care Medicine and the World Federation of Pediatric Intensive and Critical Care Societies, vol. 4, no. 2, Apr. 2003, pp. 233–38. Epmc, doi:10.1097/01.pcc.0000059729.21375.d0.
Rotta AT, Gunnarsson B, Fuhrman BP, Wiryawan B, Hernan LJ, Steinhorn DM. Perfluorooctyl bromide (perflubron) attenuates oxidative injury to biological and nonbiological systems. Pediatric critical care medicine : a journal of the Society of Critical Care Medicine and the World Federation of Pediatric Intensive and Critical Care Societies. 2003 Apr;4(2):233–238.

Published In

Pediatric critical care medicine : a journal of the Society of Critical Care Medicine and the World Federation of Pediatric Intensive and Critical Care Societies

DOI

ISSN

1529-7535

Publication Date

April 2003

Volume

4

Issue

2

Start / End Page

233 / 238

Related Subject Headings

  • Rats
  • Pulmonary Artery
  • Pediatrics
  • Oxidative Stress
  • Muscle, Smooth, Vascular
  • Liquid Ventilation
  • Lipid Peroxidation
  • Linoleic Acid
  • Hydrocarbons, Brominated
  • Fluorocarbons