Wnt traffic from endoplasmic reticulum to filopodia.

Published online

Journal Article

Wnts are a family of secreted palmitoleated glycoproteins that play key roles in cell to cell communication during development and regulate stem cell compartments in adults. Wnt receptors, downstream signaling cascades and target pathways have been extensively studied while less is known about how Wnts are secreted and move from producing cells to receiving cells. We used the synchronization system called Retention Using Selective Hook (RUSH) to study Wnt trafficking from endoplasmic reticulum to Golgi and then to plasma membrane and filopodia in real time. Inhibition of porcupine (PORCN) or knockout of Wntless (WLS) blocked Wnt exit from the ER. Wnt-containing vesicles paused at sub-cortical regions of the plasma membrane before exiting the cell. Wnt-containing vesicles were associated with filopodia extending to adjacent cells. These data visualize and confirm the role of WLS and PORCN in ER exit of Wnts and support the role of filopodia in Wnt signaling.

Full Text

Duke Authors

Cited Authors

  • Moti, N; Yu, J; Boncompain, G; Perez, F; Virshup, DM

Published Date

  • 2019

Published In

Volume / Issue

  • 14 / 2

Start / End Page

  • e0212711 -

PubMed ID

  • 30794657

Pubmed Central ID

  • 30794657

Electronic International Standard Serial Number (EISSN)

  • 1932-6203

Digital Object Identifier (DOI)

  • 10.1371/journal.pone.0212711

Language

  • eng

Conference Location

  • United States