Scalable Production of Therapeutic Protein Nanoparticles Using Flash Nanoprecipitation.


Journal Article

Flash nanoprecipitation (FNP) by fast mixing of drug-containing organic solvent and water in a microchamber is a powerful and scalable technology to produce solid drug nanoparticles with high payload. The embedded therapeutic drugs, however, are largely limited to hydrophobic small molecules. By transferring proteins into organic solvent via hydrophobic ion pairing, the scope of FNP applications is expanded. This platform technology is capable of producing protein nanoparticles with tunable sizes (from ≈30 nm to sub-micrometers), high-production scale (grams per hour), high drug loading efficiency (>90%), and excellent reproducibility, opening a new paradigm for formulation of biological pharmaceuticals. As a proof-of-concept, insulin nanoparticles are made to address a major medical challenge; oral administration. A relative insulin bioavailability of 13.2% is achieved, enabling sustained reduction of blood glucose levels in a diabetic rat model.

Full Text

Cited Authors

  • Zeng, Z; Dong, C; Zhao, P; Liu, Z; Liu, L; Mao, H-Q; Leong, KW; Gao, X; Chen, Y

Published Date

  • March 2019

Published In

Volume / Issue

  • 8 / 6

Start / End Page

  • e1801010 -

PubMed ID

  • 30338666

Pubmed Central ID

  • 30338666

Electronic International Standard Serial Number (EISSN)

  • 2192-2659

International Standard Serial Number (ISSN)

  • 2192-2640

Digital Object Identifier (DOI)

  • 10.1002/adhm.201801010


  • eng