Determination of neutralization activities by a new versatile assay using an HIV-1 genome carrying the Gaussia luciferase gene.

Published

Journal Article

Characterization of neutralizing activities are critical to evaluation of the neutralization potency and breadth of monoclonal antibodies or anti-HIV-1 sera elicited during natural HIV-1 infection or by vaccines. We have developed a new neutralization method using the SG3Δenv genome carrying the Gaussia luciferase gene between the env and nef genes. Pseudotype viruses generated using this new SG3Δenv-GLuc backbone together with HIV-1 env genes were infectious to TZM-bl cells, T cell lines and primary T cells. Viral infection can be detected by measuring luciferase activities with both lysed cells and culture supernatants. Neutralization titers in sera from HIV-1-infected individuals against tier 1 and tier 2 viruses were comparable to those determined by the gold standard TZM-bl-firefly method. Since the neutralization activities can be determined by repeatedly measuring luciferase activities in culture supernatants of any cells that are infected by SG3Δenv-GLuc-Env pseudotype viruses, this new method can serve as a versatile and high throughput assay to determine neutralization activities.

Full Text

Duke Authors

Cited Authors

  • Zhang, Y; Wang, C; Gao, N; Zhang, X; Yu, X; Xu, J; Gao, F

Published Date

  • May 2019

Published In

Volume / Issue

  • 267 /

Start / End Page

  • 22 - 28

PubMed ID

  • 30817948

Pubmed Central ID

  • 30817948

Electronic International Standard Serial Number (EISSN)

  • 1879-0984

Digital Object Identifier (DOI)

  • 10.1016/j.jviromet.2019.02.009

Language

  • eng

Conference Location

  • Netherlands