Anti-PEG Antibodies Inhibit the Anticoagulant Activity of PEGylated Aptamers.

Published

Journal Article

Biopharmaceuticals have become increasingly attractive therapeutic agents and are often PEGylated to enhance their pharmacokinetics and reduce their immunogenicity. However, recent human clinical trials have demonstrated that administration of PEGylated compounds can evoke anti-PEG antibodies. Considering the ubiquity of PEG in commercial products and the presence of pre-existing anti-PEG antibodies in patients in large clinical trials evaluating a PEG-modified aptamer, we investigated how anti-PEG antibodies effect the therapeutic activities of PEGylated RNA aptamers. We demonstrate that anti-PEG antibodies can directly bind to and inhibit anticoagulant aptamer function in vitro and in vivo. Moreover, in parallel studies we detected the presence of anti-PEG antibodies in nonhuman primates after a single administration of a PEGylated aptamer. Our results suggest that anti-PEG antibodies can limit the activity of PEGylated drugs and potentially compromise the activity of otherwise effective therapeutic agents.

Full Text

Duke Authors

Cited Authors

  • Moreno, A; Pitoc, GA; Ganson, NJ; Layzer, JM; Hershfield, MS; Tarantal, AF; Sullenger, BA

Published Date

  • May 16, 2019

Published In

Volume / Issue

  • 26 / 5

Start / End Page

  • 634 - 644.e3

PubMed ID

  • 30827937

Pubmed Central ID

  • 30827937

Electronic International Standard Serial Number (EISSN)

  • 2451-9448

Digital Object Identifier (DOI)

  • 10.1016/j.chembiol.2019.02.001

Language

  • eng

Conference Location

  • United States