Pseudocatalytic Antiaggregation Activity of Antibodies: Immunoglobulins can Influence α-Synuclein Aggregation at Substoichiometric Concentrations.

Published

Journal Article

Protein aggregation is involved in a variety of diseases. Alteration of the aggregation pathway, either to produce less toxic structures or to increase aggregate clearance, is a promising therapeutic route. Both active and passive immunization has been used for this purpose. However, the mechanism of action of antibodies on protein aggregates is not completely clear especially given poor ability of antibodies to cross blood-brain barrier. Here, we have shown that antibodies can interfere with protein aggregation at substoichiometric concentrations (as low as 1:1000 antibody to protein ratio). This is an indication that antibodies interact with aggregation intermediates in chaperone-like manner altering the aggregation pathways at very low antibody levels. This observation supports earlier suggestions that antibodies can inhibit aggregation by interaction with low abundance aggregation intermediates.

Full Text

Cited Authors

  • Breydo, L; Morgan, D; Uversky, VN

Published Date

  • April 2016

Published In

Volume / Issue

  • 53 / 3

Start / End Page

  • 1949 - 1958

PubMed ID

  • 25833100

Pubmed Central ID

  • 25833100

Electronic International Standard Serial Number (EISSN)

  • 1559-1182

International Standard Serial Number (ISSN)

  • 0893-7648

Digital Object Identifier (DOI)

  • 10.1007/s12035-015-9148-8

Language

  • eng