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Insulin-like growth factor-I is a serum component stimulating growth of human neuroblastoma.

Publication ,  Journal Article
Burke, TW; Vuk-Pavlović, S
Published in: In vitro cellular & developmental biology. Animal
May 1993

Human non-autocrine neuroblastoma cells SK-N-SH and LF require serum for proliferation in vitro. We wished to determine the role of serum-borne insulin-like growth factor I (IGF-I) as mitogen for these cells. Introduction of the monoclonal antibody alpha-IR3 against human IGF-I receptor reduced proliferation in the presence of fetal bovine serum (FBS). IGF-I (5 nM) was as effective as FBS (10%) in stimulating proliferation. Porcine insulin mimicked the effects of IGF-I, but at a 1000-fold higher concentration. The antibody alpha-IR3 reduced growth stimulated by IGF-I more effectively than growth stimulated by insulin. Thus, proliferation of human non-autocrine neuroblastoma cells can be effectively manipulated by exogenous IGF-I.

Duke Scholars

Published In

In vitro cellular & developmental biology. Animal

DOI

EISSN

1543-706X

ISSN

1071-2690

Publication Date

May 1993

Volume

29A

Issue

5

Start / End Page

391 / 394

Related Subject Headings

  • Tumor Cells, Cultured
  • Time Factors
  • Neuroblastoma
  • Kinetics
  • Insulin-Like Growth Factor I
  • Humans
  • Culture Media, Serum-Free
  • Culture Media
  • Cell Division
  • Biochemistry & Molecular Biology
 

Citation

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Burke, T. W., & Vuk-Pavlović, S. (1993). Insulin-like growth factor-I is a serum component stimulating growth of human neuroblastoma. In Vitro Cellular & Developmental Biology. Animal, 29A(5), 391–394. https://doi.org/10.1007/bf02633987
Burke, T. W., and S. Vuk-Pavlović. “Insulin-like growth factor-I is a serum component stimulating growth of human neuroblastoma.In Vitro Cellular & Developmental Biology. Animal 29A, no. 5 (May 1993): 391–94. https://doi.org/10.1007/bf02633987.
Burke TW, Vuk-Pavlović S. Insulin-like growth factor-I is a serum component stimulating growth of human neuroblastoma. In vitro cellular & developmental biology Animal. 1993 May;29A(5):391–4.
Burke, T. W., and S. Vuk-Pavlović. “Insulin-like growth factor-I is a serum component stimulating growth of human neuroblastoma.In Vitro Cellular & Developmental Biology. Animal, vol. 29A, no. 5, May 1993, pp. 391–94. Epmc, doi:10.1007/bf02633987.
Burke TW, Vuk-Pavlović S. Insulin-like growth factor-I is a serum component stimulating growth of human neuroblastoma. In vitro cellular & developmental biology Animal. 1993 May;29A(5):391–394.
Journal cover image

Published In

In vitro cellular & developmental biology. Animal

DOI

EISSN

1543-706X

ISSN

1071-2690

Publication Date

May 1993

Volume

29A

Issue

5

Start / End Page

391 / 394

Related Subject Headings

  • Tumor Cells, Cultured
  • Time Factors
  • Neuroblastoma
  • Kinetics
  • Insulin-Like Growth Factor I
  • Humans
  • Culture Media, Serum-Free
  • Culture Media
  • Cell Division
  • Biochemistry & Molecular Biology