Insulin-like growth factor-I is a serum component stimulating growth of human neuroblastoma.

Journal Article (Journal Article)

Human non-autocrine neuroblastoma cells SK-N-SH and LF require serum for proliferation in vitro. We wished to determine the role of serum-borne insulin-like growth factor I (IGF-I) as mitogen for these cells. Introduction of the monoclonal antibody alpha-IR3 against human IGF-I receptor reduced proliferation in the presence of fetal bovine serum (FBS). IGF-I (5 nM) was as effective as FBS (10%) in stimulating proliferation. Porcine insulin mimicked the effects of IGF-I, but at a 1000-fold higher concentration. The antibody alpha-IR3 reduced growth stimulated by IGF-I more effectively than growth stimulated by insulin. Thus, proliferation of human non-autocrine neuroblastoma cells can be effectively manipulated by exogenous IGF-I.

Full Text

Duke Authors

Cited Authors

  • Burke, TW; Vuk-Pavlović, S

Published Date

  • May 1993

Published In

Volume / Issue

  • 29A / 5

Start / End Page

  • 391 - 394

PubMed ID

  • 8314733

Electronic International Standard Serial Number (EISSN)

  • 1543-706X

International Standard Serial Number (ISSN)

  • 1071-2690

Digital Object Identifier (DOI)

  • 10.1007/bf02633987


  • eng