Visualizing RNA Conformational Changes via Pattern Recognition of RNA by Small Molecules.

Published

Journal Article

Conformational changes in RNA play vital roles in the regulation of many biological systems, yet these changes can be challenging to visualize. Previously, we demonstrated that Pattern Recognition of RNA by Small Molecules (PRRSM) can unbiasedly cluster defined RNA secondary structure motifs utilizing an aminoglycoside receptor library. In this work, we demonstrate the power of this method to visualize changes in folding at the secondary structure level within two distinct riboswitch structures. After labeling at three independent positions on each riboswitch, PRRSM accurately classified all apo and ligand-bound riboswitch structures, including changes in the size of a structural motif, and revealed modification sites that prevented folding and/or led to a mixture of states. These data underscore the utility and robustness of the PRRSM assay for rapid assessment of RNA structural changes and for gaining ready insight into nucleotide positions critical to RNA folding.

Full Text

Duke Authors

Cited Authors

  • Eubanks, CS; Zhao, B; Patwardhan, NN; Thompson, RD; Zhang, Q; Hargrove, AE

Published Date

  • April 2019

Published In

Volume / Issue

  • 141 / 14

Start / End Page

  • 5692 - 5698

PubMed ID

  • 30860826

Pubmed Central ID

  • 30860826

Electronic International Standard Serial Number (EISSN)

  • 1520-5126

International Standard Serial Number (ISSN)

  • 0002-7863

Digital Object Identifier (DOI)

  • 10.1021/jacs.8b09665

Language

  • eng