High-resolution intracellular recordings using a real-time computational model of the electrode.


Journal Article

Intracellular recordings of neuronal membrane potential are a central tool in neurophysiology. In many situations, especially in vivo, the traditional limitation of such recordings is the high electrode resistance and capacitance, which may cause significant measurement errors during current injection. We introduce a computer-aided technique, Active Electrode Compensation (AEC), based on a digital model of the electrode interfaced in real time with the electrophysiological setup. The characteristics of this model are first estimated using white noise current injection. The electrode and membrane contribution are digitally separated, and the recording is then made by online subtraction of the electrode contribution. Tests performed in vitro and in vivo demonstrate that AEC enables high-frequency recordings in demanding conditions, such as injection of conductance noise in dynamic-clamp mode, not feasible with a single high-resistance electrode until now. AEC should be particularly useful to characterize fast neuronal phenomena intracellularly in vivo.

Full Text

Duke Authors

Cited Authors

  • Brette, R; Piwkowska, Z; Monier, C; Rudolph-Lilith, M; Fournier, J; Levy, M; Frégnac, Y; Bal, T; Destexhe, A

Published Date

  • August 14, 2008

Published In

Volume / Issue

  • 59 / 3

Start / End Page

  • 379 - 391

PubMed ID

  • 18701064

Pubmed Central ID

  • 18701064

Electronic International Standard Serial Number (EISSN)

  • 1097-4199

Digital Object Identifier (DOI)

  • 10.1016/j.neuron.2008.06.021


  • eng

Conference Location

  • United States