Microscopy and Cell Biology: New Methods and New Questions.

Journal Article (Review;Journal Article)

Understanding the cellular basis of human health and disease requires the spatial resolution of microscopy and the molecular-level details provided by spectroscopy. This review highlights imaging methods at the intersection of microscopy and spectroscopy with applications in cell biology. Imaging methods are divided into three broad categories: fluorescence microscopy, label-free approaches, and imaging tools that can be applied to multiple imaging modalities. Just as these imaging methods allow researchers to address new biological questions, progress in biological sciences will drive the development of new imaging methods. We highlight four topics in cell biology that illustrate the need for new imaging tools: nanoparticle-cell interactions, intracellular redox chemistry, neuroscience, and the increasing use of spheroids and organoids. Overall, our goal is to provide a brief overview of individual imaging methods and highlight recent advances in the use of microscopy for cell biology.

Full Text

Duke Authors

Cited Authors

  • Morris, JD; Payne, CK

Published Date

  • June 2019

Published In

Volume / Issue

  • 70 /

Start / End Page

  • 199 - 218

PubMed ID

  • 30883272

Electronic International Standard Serial Number (EISSN)

  • 1545-1593

International Standard Serial Number (ISSN)

  • 0066-426X

Digital Object Identifier (DOI)

  • 10.1146/annurev-physchem-042018-052527


  • eng