An artery-specific fluorescent dye for studying neurovascular coupling.

Journal Article (Journal Article)

We demonstrate that Alexa Fluor 633 hydrazide (Alexa Fluor 633) selectively labels neocortical arteries and arterioles by binding to elastin fibers. We measured sensory stimulus-evoked arteriole dilation dynamics in mouse, rat and cat visual cortex using Alexa Fluor 633 together with neuronal activity using calcium indicators or blood flow using fluorescein dextran. Arteriole dilation decreased fluorescence recorded from immediately underlying neurons, representing a potential artifact during neuronal functional imaging experiments.

Full Text

Duke Authors

Cited Authors

  • Shen, Z; Lu, Z; Chhatbar, PY; O'Herron, P; Kara, P

Published Date

  • January 22, 2012

Published In

Volume / Issue

  • 9 / 3

Start / End Page

  • 273 - 276

PubMed ID

  • 22266543

Pubmed Central ID

  • PMC3392962

Electronic International Standard Serial Number (EISSN)

  • 1548-7105

Digital Object Identifier (DOI)

  • 10.1038/nmeth.1857


  • eng

Conference Location

  • United States