A Synthetic Gene Circuit for Self-Regulating Delivery of Biologic Drugs in Engineered Tissues.


Journal Article

IMPACT STATEMENT:We engineered a synthetic transcription system based on nuclear factor kappa-light-chain-enhancer of activated B cells signaling that can attenuate the effects of the inflammatory cytokine interleukin (IL)-1α in a self-regulating manner. This system responds in a time- and dose-dependent manner to rapidly produce therapeutic levels of IL-1 receptor antagonist (IL-1Ra). The use of lentiviral gene therapy allows this system to be utilized through different transduction methods and in different cell types for a variety of applications. Broadly, this approach may be applicable in developing autoregulated biologic systems for tissue engineering and drug delivery in a range of disease applications.

Full Text

Cited Authors

  • Pferdehirt, L; Ross, AK; Brunger, JM; Guilak, F

Published Date

  • May 2019

Published In

Volume / Issue

  • 25 / 9-10

Start / End Page

  • 809 - 820

PubMed ID

  • 30968743

Pubmed Central ID

  • 30968743

Electronic International Standard Serial Number (EISSN)

  • 1937-335X

International Standard Serial Number (ISSN)

  • 1937-3341

Digital Object Identifier (DOI)

  • 10.1089/ten.tea.2019.0027


  • eng