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Organ-specific metastases obtained by culturing colorectal cancer cells on tissue-specific decellularized scaffolds.

Publication ,  Journal Article
Tian, X; Werner, ME; Roche, KC; Hanson, AD; Foote, HP; Yu, SK; Warner, SB; Copp, JA; Lara, H; Wauthier, EL; Caster, JM; Herring, LE; Zhang, L ...
Published in: Nat Biomed Eng
June 2018

Metastatic disease remains the primary cause of mortality in cancer patients. Yet the number of available in vitro models to study metastasis is limited by challenges in the recapitulation of the metastatic microenvironment in vitro, and by difficulties in maintaining colonized-tissue specificity in the expansion and maintenance of metastatic cells. Here, we show that decellularized scaffolds that retain tissue-specific extracellular-matrix components and bound signalling molecules enable, when seeded with colorectal cancer cells, the spontaneous formation of three-dimensional cell colonies that histologically, molecularly and phenotypically resemble in vivo metastases. Lung and liver metastases obtained by culturing colorectal cancer cells on, respectively, lung and liver decellularized scaffolds retained their tissue-specific tropism when injected in mice. We also found that the engineered metastases contained signet ring cells, which has not previously been observed ex vivo. A culture system with tissue-specific decellularized scaffolds represents a simple and powerful approach for the study of organ-specific cancer metastases.

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Published In

Nat Biomed Eng

DOI

EISSN

2157-846X

Publication Date

June 2018

Volume

2

Issue

6

Start / End Page

443 / 452

Location

England

Related Subject Headings

  • Tumor Cells, Cultured
  • Tissue Scaffolds
  • Neoplasm Metastasis
  • Humans
  • HT29 Cells
  • Colorectal Neoplasms
  • Cell Culture Techniques
  • Caco-2 Cells
  • 4003 Biomedical engineering
 

Citation

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Tian, X., Werner, M. E., Roche, K. C., Hanson, A. D., Foote, H. P., Yu, S. K., … Wang, A. Z. (2018). Organ-specific metastases obtained by culturing colorectal cancer cells on tissue-specific decellularized scaffolds. Nat Biomed Eng, 2(6), 443–452. https://doi.org/10.1038/s41551-018-0231-0
Tian, Xi, Michael E. Werner, Kyle C. Roche, Ariel D. Hanson, Henry P. Foote, Stephanie K. Yu, Samuel B. Warner, et al. “Organ-specific metastases obtained by culturing colorectal cancer cells on tissue-specific decellularized scaffolds.Nat Biomed Eng 2, no. 6 (June 2018): 443–52. https://doi.org/10.1038/s41551-018-0231-0.
Tian X, Werner ME, Roche KC, Hanson AD, Foote HP, Yu SK, et al. Organ-specific metastases obtained by culturing colorectal cancer cells on tissue-specific decellularized scaffolds. Nat Biomed Eng. 2018 Jun;2(6):443–52.
Tian, Xi, et al. “Organ-specific metastases obtained by culturing colorectal cancer cells on tissue-specific decellularized scaffolds.Nat Biomed Eng, vol. 2, no. 6, June 2018, pp. 443–52. Pubmed, doi:10.1038/s41551-018-0231-0.
Tian X, Werner ME, Roche KC, Hanson AD, Foote HP, Yu SK, Warner SB, Copp JA, Lara H, Wauthier EL, Caster JM, Herring LE, Zhang L, Tepper JE, Hsu DS, Zhang T, Reid LM, Wang AZ. Organ-specific metastases obtained by culturing colorectal cancer cells on tissue-specific decellularized scaffolds. Nat Biomed Eng. 2018 Jun;2(6):443–452.

Published In

Nat Biomed Eng

DOI

EISSN

2157-846X

Publication Date

June 2018

Volume

2

Issue

6

Start / End Page

443 / 452

Location

England

Related Subject Headings

  • Tumor Cells, Cultured
  • Tissue Scaffolds
  • Neoplasm Metastasis
  • Humans
  • HT29 Cells
  • Colorectal Neoplasms
  • Cell Culture Techniques
  • Caco-2 Cells
  • 4003 Biomedical engineering