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Contrasting Roles of Transcription Factors Spineless and EcR in the Highly Dynamic Chromatin Landscape of Butterfly Wing Metamorphosis.

Publication ,  Journal Article
van der Burg, KRL; Lewis, JJ; Martin, A; Nijhout, HF; Danko, CG; Reed, RD
Published in: Cell reports
April 2019

Development requires highly coordinated changes in chromatin accessibility in order for proper gene regulation to occur. Here, we identify factors associated with major, discrete changes in chromatin accessibility during butterfly wing metamorphosis. By combining mRNA sequencing (mRNA-seq), assay for transposase-accessible chromatin using sequencing (ATAC-seq), and machine learning analysis of motifs, we show that distinct sets of transcription factors are predictive of chromatin opening at different developmental stages. Our data suggest an important role for nuclear hormone receptors early in metamorphosis, whereas PAS-domain transcription factors are strongly associated with later chromatin opening. Chromatin immunoprecipitation sequencing (ChIP-seq) validation of select candidate factors showed spineless binding to be a major predictor of opening chromatin. Surprisingly, binding of ecdysone receptor (EcR), a candidate accessibility factor in Drosophila, was not predictive of opening but instead marked persistent sites. This work characterizes the chromatin dynamics of insect wing metamorphosis, identifies candidate chromatin remodeling factors in insects, and presents a genome assembly of the model butterfly Junonia coenia.

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Published In

Cell reports

DOI

EISSN

2211-1247

ISSN

2211-1247

Publication Date

April 2019

Volume

27

Issue

4

Start / End Page

1027 / 1038.e3

Related Subject Headings

  • Wings, Animal
  • Receptors, Steroid
  • RNA, Messenger
  • Metamorphosis, Biological
  • Machine Learning
  • Insect Proteins
  • Gene Expression Regulation, Developmental
  • Chromatin Immunoprecipitation
  • Chromatin
  • Butterflies
 

Citation

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van der Burg, K. R. L., Lewis, J. J., Martin, A., Nijhout, H. F., Danko, C. G., & Reed, R. D. (2019). Contrasting Roles of Transcription Factors Spineless and EcR in the Highly Dynamic Chromatin Landscape of Butterfly Wing Metamorphosis. Cell Reports, 27(4), 1027-1038.e3. https://doi.org/10.1016/j.celrep.2019.03.092
Burg, Karin R. L. van der, James J. Lewis, Arnaud Martin, H Frederik Nijhout, Charles G. Danko, and Robert D. Reed. “Contrasting Roles of Transcription Factors Spineless and EcR in the Highly Dynamic Chromatin Landscape of Butterfly Wing Metamorphosis.Cell Reports 27, no. 4 (April 2019): 1027-1038.e3. https://doi.org/10.1016/j.celrep.2019.03.092.
van der Burg KRL, Lewis JJ, Martin A, Nijhout HF, Danko CG, Reed RD. Contrasting Roles of Transcription Factors Spineless and EcR in the Highly Dynamic Chromatin Landscape of Butterfly Wing Metamorphosis. Cell reports. 2019 Apr;27(4):1027-1038.e3.
van der Burg, Karin R. L., et al. “Contrasting Roles of Transcription Factors Spineless and EcR in the Highly Dynamic Chromatin Landscape of Butterfly Wing Metamorphosis.Cell Reports, vol. 27, no. 4, Apr. 2019, pp. 1027-1038.e3. Epmc, doi:10.1016/j.celrep.2019.03.092.
van der Burg KRL, Lewis JJ, Martin A, Nijhout HF, Danko CG, Reed RD. Contrasting Roles of Transcription Factors Spineless and EcR in the Highly Dynamic Chromatin Landscape of Butterfly Wing Metamorphosis. Cell reports. 2019 Apr;27(4):1027-1038.e3.
Journal cover image

Published In

Cell reports

DOI

EISSN

2211-1247

ISSN

2211-1247

Publication Date

April 2019

Volume

27

Issue

4

Start / End Page

1027 / 1038.e3

Related Subject Headings

  • Wings, Animal
  • Receptors, Steroid
  • RNA, Messenger
  • Metamorphosis, Biological
  • Machine Learning
  • Insect Proteins
  • Gene Expression Regulation, Developmental
  • Chromatin Immunoprecipitation
  • Chromatin
  • Butterflies