Self-Organized Nuclear Positioning Synchronizes the Cell Cycle in Drosophila Embryos.

Published

Journal Article

The synchronous cleavage divisions of early embryogenesis require coordination of the cell-cycle oscillator, the dynamics of the cytoskeleton, and the cytoplasm. Yet, it remains unclear how spatially restricted biochemical signals are integrated with physical properties of the embryo to generate collective dynamics. Here, we show that synchronization of the cell cycle in Drosophila embryos requires accurate nuclear positioning, which is regulated by the cell-cycle oscillator through cortical contractility and cytoplasmic flows. We demonstrate that biochemical oscillations are initiated by local Cdk1 inactivation and spread through the activity of phosphatase PP1 to generate cortical myosin II gradients. These gradients cause cortical and cytoplasmic flows that control proper nuclear positioning. Perturbations of PP1 activity and optogenetic manipulations of cortical actomyosin disrupt nuclear spreading, resulting in loss of cell-cycle synchrony. We conclude that mitotic synchrony is established by a self-organized mechanism that integrates the cell-cycle oscillator and embryo mechanics.

Full Text

Duke Authors

Cited Authors

  • Deneke, VE; Puliafito, A; Krueger, D; Narla, AV; De Simone, A; Primo, L; Vergassola, M; De Renzis, S; Di Talia, S

Published Date

  • May 2, 2019

Published In

Volume / Issue

  • 177 / 4

Start / End Page

  • 925 - 941.e17

PubMed ID

  • 30982601

Pubmed Central ID

  • 30982601

Electronic International Standard Serial Number (EISSN)

  • 1097-4172

Digital Object Identifier (DOI)

  • 10.1016/j.cell.2019.03.007

Language

  • eng

Conference Location

  • United States