Genomic and Transcriptomic Profiling of Combined Hepatocellular and Intrahepatic Cholangiocarcinoma Reveals Distinct Molecular Subtypes.

Published

Journal Article

We performed genomic and transcriptomic sequencing of 133 combined hepatocellular and intrahepatic cholangiocarcinoma (cHCC-ICC) cases, including separate, combined, and mixed subtypes. Integrative comparison of cHCC-ICC with hepatocellular carcinoma and intrahepatic cholangiocarcinoma revealed that combined and mixed type cHCC-ICCs are distinct subtypes with different clinical and molecular features. Integrating laser microdissection, cancer cell fraction analysis, and single nucleus sequencing, we revealed both mono- and multiclonal origins in the separate type cHCC-ICCs, whereas combined and mixed type cHCC-ICCs were all monoclonal origin. Notably, cHCC-ICCs showed significantly higher expression of Nestin, suggesting Nestin may serve as a biomarker for diagnosing cHCC-ICC. Our results provide important biological and clinical insights into cHCC-ICC.

Full Text

Duke Authors

Cited Authors

  • Xue, R; Chen, L; Zhang, C; Fujita, M; Li, R; Yan, S-M; Ong, CK; Liao, X; Gao, Q; Sasagawa, S; Li, Y; Wang, J; Guo, H; Huang, Q-T; Zhong, Q; Tan, J; Qi, L; Gong, W; Hong, Z; Li, M; Zhao, J; Peng, T; Lu, Y; Lim, KHT; Boot, A; Ono, A; Chayama, K; Zhang, Z; Rozen, SG; Teh, BT; Wang, XW; Nakagawa, H; Zeng, M-S; Bai, F; Zhang, N

Published Date

  • June 10, 2019

Published In

Volume / Issue

  • 35 / 6

Start / End Page

  • 932 - 947.e8

PubMed ID

  • 31130341

Pubmed Central ID

  • 31130341

Electronic International Standard Serial Number (EISSN)

  • 1878-3686

Digital Object Identifier (DOI)

  • 10.1016/j.ccell.2019.04.007

Language

  • eng

Conference Location

  • United States