Functional CD3+CD8+PD1- T Cell Accumulation and PD-L1 Expression Increases During Tumor Invasion in DCIS of the Breast.

Journal Article (Journal Article)

BACKGROUND: The changes in T cell subsets and programmed death ligand 1 (PD-L1) expression during the transition from ductal carcinoma in situ (DCIS) to early invasive breast cancer had not been well studied. PATIENTS AND METHODS: A total of 85 DCIS patients were classified into 49 DCIS (clinical stage: Tis, noninvasive) and 36 with a minimally infiltrating lesion (MIL; < 5 mm; clinical stage: T1a). We explored the quantitative alterations of T-cell markers and PD-L1 in these groups using the Opal multi-immunohistochemistry technique. RESULTS: We observed increased infiltration of CD3-positive (CD3+)CD8+ programmed death 1 (PD1)-negative T cells and higher PD-L1 expression in DCIS with MIL. Elevated PD1 expression correlated with PD-L1 expression in MIL and DCIS. CONCLUSION: We conclude that during the transition from DCIS to an invasive lesion, the host cytolytic T cells begin interacting with the tumor and destroy the tumor tissue, leading to an adaptive upregulation of PD-L1 and tumor protection against immune destruction.

Full Text

Duke Authors

Cited Authors

  • Lv, S; Wang, S; Qiao, G; Wang, X; Zhou, X; Yan, F; Li, Y; Wang, S; Morse, MA; Hobeika, A; Ren, J; Lyerly, HK

Published Date

  • October 2019

Published In

Volume / Issue

  • 19 / 5

Start / End Page

  • e617 - e623

PubMed ID

  • 31101455

Electronic International Standard Serial Number (EISSN)

  • 1938-0666

Digital Object Identifier (DOI)

  • 10.1016/j.clbc.2019.04.001


  • eng

Conference Location

  • United States