Control of antiviral innate immune response by protein geranylgeranylation.

Published online

Journal Issue

The mitochondrial antiviral signaling protein (MAVS) orchestrates host antiviral innate immune response to RNA virus infection. However, how MAVS signaling is controlled to eradicate virus while preventing self-destructive inflammation remains obscure. Here, we show that protein geranylgeranylation, a posttranslational lipid modification of proteins, limits MAVS-mediated immune signaling by targeting Rho family small guanosine triphosphatase Rac1 into the mitochondria-associated endoplasmic reticulum (ER) membranes (MAMs) at the mitochondria-ER junction. Protein geranylgeranylation and subsequent palmitoylation promote Rac1 translocation into MAMs upon viral infection. MAM-localized Rac1 limits MAVS' interaction with E3 ligase Trim31 and hence inhibits MAVS ubiquitination, aggregation, and activation. Rac1 also facilitates the recruitment of caspase-8 and cFLIPL to the MAVS signalosome and the subsequent cleavage of Ripk1 that terminates MAVS signaling. Consistently, mice with myeloid deficiency of protein geranylgeranylation showed improved survival upon influenza A virus infection. Our work revealed a critical role of protein geranylgeranylation in regulating antiviral innate immune response.

Full Text

Duke Authors

Cited Authors

  • Yang, S; Harding, AT; Sweeney, C; Miao, D; Swan, G; Zhou, C; Jiang, Z; Fitzgerald, KA; Hammer, G; Bergo, MO; Kroh, HK; Lacy, DB; Sun, C; Glogauer, M; Que, LG; Heaton, NS; Wang, D

Published Date

  • May 2019

Published In

Volume / Issue

  • 5 / 5

Start / End Page

  • eaav7999 -

PubMed ID

  • 31149635

Pubmed Central ID

  • 31149635

Electronic International Standard Serial Number (EISSN)

  • 2375-2548

Digital Object Identifier (DOI)

  • 10.1126/sciadv.aav7999

Language

  • eng

Conference Location

  • United States