Engineered Ribonucleoprotein Granules Inhibit Translation in Protocells.
Journal Article (Journal Article)
Liquid granules rich in intrinsically disordered proteins and RNA play key roles in critical cellular functions such as RNA processing and translation. Many details of the mechanism via which this occurs remain to be elucidated. Motivated by the lacuna in the field and by the prospects of developing de novo artificial granules that provide extrinsic control of translation, we report a bottom-up approach to engineer ribonucleoprotein granules composed of a recombinant RNA-binding IDP that exhibits phase behavior in water. We developed a kinetic model to illustrate that these granules inhibit translation through reversible or irreversible sequestration of mRNA. Within monodisperse droplets capable of transcription and translation, we experimentally demonstrate temporal inhibition of translation by using designer IDPs that exhibit tunable phase behavior. This work lays the foundation for developing artificial granules that promise to further our mechanistic understanding of their naturally occurring counterparts.
Full Text
Duke Authors
Cited Authors
- Simon, JR; Eghtesadi, SA; Dzuricky, M; You, L; Chilkoti, A
Published Date
- July 2019
Published In
Volume / Issue
- 75 / 1
Start / End Page
- 66 - 75.e5
PubMed ID
- 31175012
Pubmed Central ID
- PMC6640848
Electronic International Standard Serial Number (EISSN)
- 1097-4164
International Standard Serial Number (ISSN)
- 1097-2765
Digital Object Identifier (DOI)
- 10.1016/j.molcel.2019.05.010
Language
- eng