Skip to main content
Journal cover image

CCR5 Signaling Promotes Murine and Human Hematopoietic Regeneration following Ionizing Radiation.

Publication ,  Journal Article
Piryani, SO; Kam, AYF; Vu, UT; Chao, NJ; Doan, PL
Published in: Stem Cell Reports
July 9, 2019

Hematopoietic stem and progenitor cells (HSPCs) depend on regulatory cytokines from the marrow microenvironment. From an unbiased cytokine screen of murine marrow supernatants, we identified C-C motif chemokine ligand 5 (CCL5) as an endothelial cell-secreted hematopoietic growth factor. Following treatment with CCL5, hematopoietic regeneration is accelerated and survival is prolonged after radiation. In mice with deletion of Ccr5, hematopoietic regeneration is delayed compared to control mice. Deletion of Ccr5 specifically in hematopoietic cells was sufficient to delay regeneration, while the deletion of Ccr5 in stromal/endothelial cells was not. Mechanistically, CCL5 promotes hematopoietic cell cycling and cell survival. Like murine hematopoietic cells, human hematopoietic cells (cord blood, healthy marrow, and peripheral blood) increase CCR5 expression after radiation exposure to promote cell survival. These data establish that CCL5 and CCR5 signaling play critical roles in hematopoietic regeneration and could serve as therapeutic targets to shorten the duration of myelosuppression.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Stem Cell Reports

DOI

EISSN

2213-6711

Publication Date

July 9, 2019

Volume

13

Issue

1

Start / End Page

76 / 90

Location

United States

Related Subject Headings

  • Signal Transduction
  • Receptors, CCR5
  • Radiation, Ionizing
  • Mice
  • Immunophenotyping
  • Humans
  • Hematopoietic Stem Cells
  • Hematopoiesis
  • Gene Expression
  • Dose-Response Relationship, Radiation
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Piryani, S. O., Kam, A. Y. F., Vu, U. T., Chao, N. J., & Doan, P. L. (2019). CCR5 Signaling Promotes Murine and Human Hematopoietic Regeneration following Ionizing Radiation. Stem Cell Reports, 13(1), 76–90. https://doi.org/10.1016/j.stemcr.2019.04.023
Piryani, Sadhna O., Angel Y. F. Kam, Uyen T. Vu, Nelson J. Chao, and Phuong L. Doan. “CCR5 Signaling Promotes Murine and Human Hematopoietic Regeneration following Ionizing Radiation.Stem Cell Reports 13, no. 1 (July 9, 2019): 76–90. https://doi.org/10.1016/j.stemcr.2019.04.023.
Piryani SO, Kam AYF, Vu UT, Chao NJ, Doan PL. CCR5 Signaling Promotes Murine and Human Hematopoietic Regeneration following Ionizing Radiation. Stem Cell Reports. 2019 Jul 9;13(1):76–90.
Piryani, Sadhna O., et al. “CCR5 Signaling Promotes Murine and Human Hematopoietic Regeneration following Ionizing Radiation.Stem Cell Reports, vol. 13, no. 1, July 2019, pp. 76–90. Pubmed, doi:10.1016/j.stemcr.2019.04.023.
Piryani SO, Kam AYF, Vu UT, Chao NJ, Doan PL. CCR5 Signaling Promotes Murine and Human Hematopoietic Regeneration following Ionizing Radiation. Stem Cell Reports. 2019 Jul 9;13(1):76–90.
Journal cover image

Published In

Stem Cell Reports

DOI

EISSN

2213-6711

Publication Date

July 9, 2019

Volume

13

Issue

1

Start / End Page

76 / 90

Location

United States

Related Subject Headings

  • Signal Transduction
  • Receptors, CCR5
  • Radiation, Ionizing
  • Mice
  • Immunophenotyping
  • Humans
  • Hematopoietic Stem Cells
  • Hematopoiesis
  • Gene Expression
  • Dose-Response Relationship, Radiation