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Transcriptome profiling reveals Th2 bias and identifies endogenous itch mediators in poison ivy contact dermatitis.

Publication ,  Journal Article
Liu, B; Tai, Y; Liu, B; Caceres, AI; Yin, C; Jordt, S-E
Published in: JCI Insight
June 11, 2019

In the United States, poison ivy exposure is the most common naturally occurring allergen to cause allergic contact dermatitis (ACD). The immune and pruritic mechanisms associated with poison ivy ACD remain largely unexplored. Here, we compared skin whole transcriptomes and itch mediator levels in mouse ACD models induced by the poison ivy allergen, urushiol, and the synthetic allergen, oxazolone. The urushiol model produced a Th2-biased immune response and scratching behavior, resembling findings in poison ivy patients. Urushiol-challenged skin contained elevated levels of the cytokine thymic stromal lymphopoietin (TSLP), a T-cell regulator and itch mediator, and pruritogenic serotonin (5-HT) and endothelin (ET-1), but not substance P (SP) or histamine. The oxazolone model generated a mixed Th1/Th2 response associated with increased levels of substance P, 5-HT, ET-1, but not TSLP or histamine. Injections of a TSLP monoclonal neutralizing antibody, serotonergic or endothelin inhibitors, but not SP inhibitors or antihistamines, reduced scratching behaviors in urushiol-challenged mice. Our findings suggest that the mouse urushiol model may serve as a translational model of human poison ivy ACD study. Inhibiting signaling by TSLP and other cytokines may represent alternatives to the standard steroid/antihistamine regimen for steroid-resistant or -intolerant patients and in exaggerated systemic responses to poison ivy.

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Published In

JCI Insight

DOI

EISSN

2379-3708

Publication Date

June 11, 2019

Volume

5

Issue

14

Location

United States

Related Subject Headings

  • Toxicodendron
  • Thymic Stromal Lymphopoietin
  • Th2 Cells
  • Skin
  • Signal Transduction
  • Pruritus
  • Oxazolone
  • Mice
  • Male
  • Inflammation Mediators
 

Citation

APA
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Liu, B., Tai, Y., Caceres, A. I., Yin, C., & Jordt, S.-E. (2019). Transcriptome profiling reveals Th2 bias and identifies endogenous itch mediators in poison ivy contact dermatitis. JCI Insight, 5(14). https://doi.org/10.1172/jci.insight.124497
Liu, Boyi, Yan Tai, Boyu Liu, Ana I. Caceres, Chengyu Yin, and Sven-Eric Jordt. “Transcriptome profiling reveals Th2 bias and identifies endogenous itch mediators in poison ivy contact dermatitis.JCI Insight 5, no. 14 (June 11, 2019). https://doi.org/10.1172/jci.insight.124497.
Liu, Boyi, et al. “Transcriptome profiling reveals Th2 bias and identifies endogenous itch mediators in poison ivy contact dermatitis.JCI Insight, vol. 5, no. 14, June 2019. Pubmed, doi:10.1172/jci.insight.124497.

Published In

JCI Insight

DOI

EISSN

2379-3708

Publication Date

June 11, 2019

Volume

5

Issue

14

Location

United States

Related Subject Headings

  • Toxicodendron
  • Thymic Stromal Lymphopoietin
  • Th2 Cells
  • Skin
  • Signal Transduction
  • Pruritus
  • Oxazolone
  • Mice
  • Male
  • Inflammation Mediators