Identification of Aedes aegypti and its respective life stages by real-time polymerase chain reaction.

Journal Article (Journal Article)

An Aedes aegypti-specific, fluorogenic probe hydrolysis (Taq-Man), polymerase chain reaction assay was developed for real-time screening using a field-deployable thermocycler. Laboratory-based testing of A. aegypti, A. aegypti (Trinidad strain), Culex pipiens, Culex quinquefasciatus, Anopheles stephensi, and Ochlerotatus taeniorhynchus individual adult mosquitoes and mixed pools (n = 10) demonstrated 100% concordance in both in vitro sensitivity (six of six samples) and specificity (10 of 10 samples). A single adult A. aegypti was identified in a pool of 100 non-A. aegypti mosquitoes. The limit of detection of A. aegypti egg pools was five individual eggs. Field testing was conducted in central Honduras. An A. aegypti and Culex spp. panel of individual and mixed pools (n = 30) of adult mosquitoes, pupae, and larvae demonstrated 100% concordance in sensitivity (22 of 22 samples) and 97% concordance in specificity (29 of 30 samples), with one false-positive result. Field testing of an A. aegypti and Culex spp. blind panel (n = 16) consisting of individual and mixed pools of adult mosquitoes, pupae, and larvae demonstrated 90% concordance in sensitivity (nine of 10 samples) and 88% concordance in specificity (14 of 16 samples).

Full Text

Duke Authors

Cited Authors

  • McAvin, JC; Bowles, DE; Swaby, JA; Blount, KW; Blow, JA; Quintana, M; Hickman, JR; Atchley, DH; Niemeyer, DM

Published Date

  • December 2005

Published In

Volume / Issue

  • 170 / 12

Start / End Page

  • 1060 - 1065

PubMed ID

  • 16491948

International Standard Serial Number (ISSN)

  • 0026-4075

Digital Object Identifier (DOI)

  • 10.7205/milmed.170.12.1060


  • eng

Conference Location

  • England