Rapid identification of dengue virus by reverse transcription-polymerase chain reaction using field-deployable instrumentation.

Published

Journal Article

Dengue virus universal and dengue serotype 1 to 4, fluorogenic probe hydrolysis (TaqMan), reverse transcription-polymerase chain reaction assays were developed for screening and serotype identification of infected mosquito vectors and human sera using a field-deployable, fluorometric thermocycler. Dengue universal and dengue 1 to 4 serotype assay in vitro sensitivity and specificity results were 100% concordant when tested with total nucleic acid extracts of multiple strains of dengue serotype 1 to 4, yellow fever, Japanese encephalitis, West Nile, and St. Louis encephalitis viruses. The in vitro sensitivity and specificity results for all five assays were concordant when tested with a blind panel of 27 dengue virus-infected mosquitoes, 21 non-dengue (yellow fever, West Nile, or St. Louis encephalitis) flavivirus-infected mosquitoes, and 11 uninfected mosquitoes and with clinical specimens consisting of a human serum panel of eight dengue viremic and 31 non-dengue-infected febrile patient serum samples. No cross-reaction occurred with vector species or human genomic DNA. Sample processing and polymerase chain reaction required <2 hours.

Full Text

Duke Authors

Cited Authors

  • McAvin, JC; Escamilla, EM; Blow, JA; Turell, MJ; Quintana, M; Bowles, DE; Swaby, JA; Barnes, WJ; Huff, WB; Lohman, KL; Atchley, DH; Hickman, JR; Niemeyer, DM

Published Date

  • December 2005

Published In

Volume / Issue

  • 170 / 12

Start / End Page

  • 1053 - 1059

PubMed ID

  • 16491947

Pubmed Central ID

  • 16491947

International Standard Serial Number (ISSN)

  • 0026-4075

Digital Object Identifier (DOI)

  • 10.7205/milmed.170.12.1053

Language

  • eng

Conference Location

  • England