Rapid identification of dengue virus by reverse transcription-polymerase chain reaction using field-deployable instrumentation.
Journal Article (Journal Article)
Dengue virus universal and dengue serotype 1 to 4, fluorogenic probe hydrolysis (TaqMan), reverse transcription-polymerase chain reaction assays were developed for screening and serotype identification of infected mosquito vectors and human sera using a field-deployable, fluorometric thermocycler. Dengue universal and dengue 1 to 4 serotype assay in vitro sensitivity and specificity results were 100% concordant when tested with total nucleic acid extracts of multiple strains of dengue serotype 1 to 4, yellow fever, Japanese encephalitis, West Nile, and St. Louis encephalitis viruses. The in vitro sensitivity and specificity results for all five assays were concordant when tested with a blind panel of 27 dengue virus-infected mosquitoes, 21 non-dengue (yellow fever, West Nile, or St. Louis encephalitis) flavivirus-infected mosquitoes, and 11 uninfected mosquitoes and with clinical specimens consisting of a human serum panel of eight dengue viremic and 31 non-dengue-infected febrile patient serum samples. No cross-reaction occurred with vector species or human genomic DNA. Sample processing and polymerase chain reaction required <2 hours.
Full Text
Duke Authors
Cited Authors
- McAvin, JC; Escamilla, EM; Blow, JA; Turell, MJ; Quintana, M; Bowles, DE; Swaby, JA; Barnes, WJ; Huff, WB; Lohman, KL; Atchley, DH; Hickman, JR; Niemeyer, DM
Published Date
- December 2005
Published In
Volume / Issue
- 170 / 12
Start / End Page
- 1053 - 1059
PubMed ID
- 16491947
International Standard Serial Number (ISSN)
- 0026-4075
Digital Object Identifier (DOI)
- 10.7205/milmed.170.12.1053
Language
- eng
Conference Location
- England