Prion disease diagnosis by proteomic profiling.

Journal Article (Journal Article)

Definitive prion disease diagnosis is currently limited to postmortem assay for the presence of the disease-associated proteinase K-resistant prion protein. Using cerebrospinal fluid (CSF) from prion-infected hamsters, matrix-assisted laser desorption/ionization Fourier transform mass spectrometry (MALDI-FTMS), and support vector machines (SVM), we have identified peptide profiles characteristic of disease state. Using 10-fold leave-one-out cross-validation, we report a predictive accuracy of 72% with a true positive rate of 73% and a false positive rate of 27% demonstrating the suitability of using proteomic profiling and CSF for the development of multiple marker diagnostics of prion disease.

Full Text

Duke Authors

Cited Authors

  • Herbst, A; McIlwain, S; Schmidt, JJ; Aiken, JM; Page, CD; Li, L

Published Date

  • February 2009

Published In

Volume / Issue

  • 8 / 2

Start / End Page

  • 1030 - 1036

PubMed ID

  • 19133784

Pubmed Central ID

  • PMC2652651

International Standard Serial Number (ISSN)

  • 1535-3893

Digital Object Identifier (DOI)

  • 10.1021/pr800832s


  • eng

Conference Location

  • United States