Impact of synchronized anti-PD-1 with Ad-CEA vaccination on inhibition of colon cancer growth.

Published

Journal Article

Aim: The purpose of this study was to determine whether addition of anti-PD-1 antibody increased the immunogenicity and anti-tumor activity of Ad-CEA vaccination in a murine model of colon cancer. Methods: Ad-CEA was administered prior to implantation of MC-38-CEA cells followed by administration of anti-PD-1 antibody. CEA-specific T-cell responses were measured by flow cytometry and ELISPOT. Dynamic co-culture of splenocytes with tumor cells was conducted to analyze anti-tumor activities. Tumor infiltration by lymphocytes was measured by IHC. Tumor volume and overall survival were also recorded. Results: Ad-CEA combined with anti-PD-1 antibody showed greater anti-tumor activity compared with either alone. The combination also increased T-cell infiltration but decreased Tregs. Conclusion: Combining Ad-CEA vaccination with anti-PD-1 antibody enhanced anti-tumor activity and immune responses.

Full Text

Duke Authors

Cited Authors

  • Sun, Y; Wang, S; Yang, H; Wu, J; Li, S; Qiao, G; Wang, S; Wang, X; Zhou, X; Osada, T; Hobeika, A; Morse, MA; Ren, J; Lyerly, HK

Published Date

  • August 2019

Published In

Volume / Issue

  • 11 / 11

Start / End Page

  • 953 - 966

PubMed ID

  • 31192764

Pubmed Central ID

  • 31192764

Electronic International Standard Serial Number (EISSN)

  • 1750-7448

Digital Object Identifier (DOI)

  • 10.2217/imt-2019-0055

Language

  • eng

Conference Location

  • England