Impact of synchronized anti-PD-1 with Ad-CEA vaccination on inhibition of colon cancer growth.
Journal Article (Journal Article)
Aim: The purpose of this study was to determine whether addition of anti-PD-1 antibody increased the immunogenicity and anti-tumor activity of Ad-CEA vaccination in a murine model of colon cancer. Methods: Ad-CEA was administered prior to implantation of MC-38-CEA cells followed by administration of anti-PD-1 antibody. CEA-specific T-cell responses were measured by flow cytometry and ELISPOT. Dynamic co-culture of splenocytes with tumor cells was conducted to analyze anti-tumor activities. Tumor infiltration by lymphocytes was measured by IHC. Tumor volume and overall survival were also recorded. Results: Ad-CEA combined with anti-PD-1 antibody showed greater anti-tumor activity compared with either alone. The combination also increased T-cell infiltration but decreased Tregs. Conclusion: Combining Ad-CEA vaccination with anti-PD-1 antibody enhanced anti-tumor activity and immune responses.
- Sun, Y; Wang, S; Yang, H; Wu, J; Li, S; Qiao, G; Wang, S; Wang, X; Zhou, X; Osada, T; Hobeika, A; Morse, MA; Ren, J; Lyerly, HK
- August 2019
Volume / Issue
- 11 / 11
Start / End Page
- 953 - 966
Electronic International Standard Serial Number (EISSN)
Digital Object Identifier (DOI)