Characterization of Acyl Carrier Protein-Dependent Glycosyltransferase in Mitomycin C Biosynthesis.

Journal Article (Journal Article)

Mitomycins make up a group of antitumor natural products that are biosynthesized from aminohydroxybenzoic acid (AHBA) and N-acetylglucosamine (GlcNAc). While the biosynthetic gene cluster was reported two decades ago, the mechanism by which the two building blocks, AHBA and GlcNAc, are coupled during biosynthesis remained uncharacterized. Here we report evidence that AHBA is first loaded onto an MmcB acyl carrier protein (ACP) by a MitE acyl ACP synthetase, followed by a transfer of GlcNAc from UDP-GlcNAc by MitB. The results suggest that the early steps of mitomycin biosynthesis proceed via intermediates linked to MmcB.

Full Text

Duke Authors

Cited Authors

  • Nguyen, HP; Yokoyama, K

Published Date

  • June 25, 2019

Published In

Volume / Issue

  • 58 / 25

Start / End Page

  • 2804 - 2808

PubMed ID

  • 31188570

Pubmed Central ID

  • PMC6597268

Electronic International Standard Serial Number (EISSN)

  • 1520-4995

Digital Object Identifier (DOI)

  • 10.1021/acs.biochem.9b00379

Language

  • eng

Conference Location

  • United States