Gut organoids: mini-tissues in culture to study intestinal physiology and disease.

Published

Journal Article

In vitro, cell cultures are essential tools in the study of intestinal function and disease. For the past few decades, monolayer cellular cultures, such as cancer cell lines or immortalized cell lines, have been widely applied in gastrointestinal research. Recently, the development of three-dimensional cultures known as organoids has permitted the growth of normal crypt-villus units that recapitulate many aspects of intestinal physiology. Organoid culturing has also been applied to study gastrointestinal diseases, intestinal-microbe interactions, and colorectal cancer. These models are amenable to CRISPR gene editing and drug treatments, including high-throughput small-molecule testing. Three-dimensional intestinal cultures have been transplanted into mice to develop versatile in vivo models of intestinal disease, particularly cancer. Limitations of currently available organoid models include cost and challenges in modeling nonepithelial intestinal cells, such as immune cells and the microbiota. Here, we describe the development of organoid models of intestinal biology and the applications of organoids for study of the pathophysiology of intestinal diseases and cancer.

Full Text

Duke Authors

Cited Authors

  • Almeqdadi, M; Mana, MD; Roper, J; Yilmaz, ÖH

Published Date

  • September 1, 2019

Published In

Volume / Issue

  • 317 / 3

Start / End Page

  • C405 - C419

PubMed ID

  • 31216420

Pubmed Central ID

  • 31216420

Electronic International Standard Serial Number (EISSN)

  • 1522-1563

Digital Object Identifier (DOI)

  • 10.1152/ajpcell.00300.2017

Language

  • eng

Conference Location

  • United States