The roles of structural dynamics in the cellular functions of RNAs.


Journal Article (Review)

RNAs fold into 3D structures that range from simple helical elements to complex tertiary structures and quaternary ribonucleoprotein assemblies. The functions of many regulatory RNAs depend on how their 3D structure changes in response to a diverse array of cellular conditions. In this Review, we examine how the structural characterization of RNA as dynamic ensembles of conformations, which form with different probabilities and at different timescales, is improving our understanding of RNA function in cells. We discuss the mechanisms of gene regulation by microRNAs, riboswitches, ribozymes, post-transcriptional RNA modifications and RNA-binding proteins, and how the cellular environment and processes such as liquid-liquid phase separation may affect RNA folding and activity. The emerging RNA-ensemble-function paradigm is changing our perspective and understanding of RNA regulation, from in vitro to in vivo and from descriptive to predictive.

Full Text

Duke Authors

Cited Authors

  • Ganser, LR; Kelly, ML; Herschlag, D; Al-Hashimi, HM

Published Date

  • August 2019

Published In

Volume / Issue

  • 20 / 8

Start / End Page

  • 474 - 489

PubMed ID

  • 31182864

Pubmed Central ID

  • 31182864

Electronic International Standard Serial Number (EISSN)

  • 1471-0080

Digital Object Identifier (DOI)

  • 10.1038/s41580-019-0136-0


  • eng

Conference Location

  • England