Formins regulate the actin-related protein 2/3 complex-independent polarization of the centrosome to the immunological synapse.

Published

Journal Article

T cell receptor (TCR)-mediated cytoskeletal reorganization is considered to be actin-related protein (Arp) 2/3 complex dependent. We therefore examined the requirement for Arp2/3- and formin-dependent F-actin nucleation during T cell activation. We demonstrated that without Arp2/3-mediated actin nucleation, stimulated T cells could not form an F-actin-rich lamellipod, but instead produced polarized filopodia-like structures. Moreover, the microtubule-organizing center (MTOC, or centrosome), which rapidly reorients to the immunological synapse through an unknown mechanism, polarized in the absence of Arp2/3. Conversely, the actin-nucleating formins, Diaphanous-1 (DIA1) and Formin-like-1 (FMNL1), did not affect TCR-stimulated F-actin-rich structures, but instead displayed unique patterns of centrosome colocalization and controlled TCR-mediated centrosome polarization. Depletion of FMNL1 or DIA1 in cytotoxic lymphocytes abrogated cell-mediated killing. Altogether, our results have identified Arp2/3 complex-independent cytoskeletal reorganization events in T lymphocytes and indicate that formins are essential cytoskeletal regulators of centrosome polarity in T cells.

Full Text

Duke Authors

Cited Authors

  • Gomez, TS; Kumar, K; Medeiros, RB; Shimizu, Y; Leibson, PJ; Billadeau, DD

Published Date

  • February 2007

Published In

Volume / Issue

  • 26 / 2

Start / End Page

  • 177 - 190

PubMed ID

  • 17306570

Pubmed Central ID

  • 17306570

International Standard Serial Number (ISSN)

  • 1074-7613

Digital Object Identifier (DOI)

  • 10.1016/j.immuni.2007.01.008

Language

  • eng

Conference Location

  • United States