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Enhancing Schwann cell migration using concentration gradients of laminin-derived peptides.

Publication ,  Journal Article
Motta, CMM; Endres, KJ; Wesdemiotis, C; Willits, RK; Becker, ML
Published in: Biomaterials
October 2019

Neuroregeneration following peripheral nerve injury is largely mediated by Schwann cells (SC), the principal glial cell that supports neurons in the peripheral nervous system. Axonal regeneration in vivo is limited by the extent of SC migration into the gap between the proximal and distal nerve, however, little is known regarding the principal driving forces for SC migration. Engineered microenvironments, such as molecular and protein gradients, play a role in the migration of many cell types, including cancer cells and fibroblasts. However, haptotactic strategies have not been applied widely to SC. Herein, a series of tethered laminin-derived peptides were analyzed for their influence on SC adhesion, proliferation, and alignment. Concentration gradient substrates were fabricated using a controlled vapor deposition method, followed by covalent peptide attachment via a thiol-ene reaction, and characterized by X-ray photoelectron spectroscopy (XPS) and MALDI-MS imaging. While tethered RGD peptides supported SC adhesion and proliferation, concentration gradients of RGD had little influence on biased SC directional migration. In contrast, YIGSR promoted less SC attachment than RGD, yet YIGSR peptide gradients directed migration with a strong bias to the concentration profile. With YIGSR peptide, overall speed increased with the steepness of the peptide concentration profile. YIGSR gradients had no haptotactic effect on rat dermal fibroblast migration, in contrast to fibroblast migration on RGD gradients. The response of SC to these tethered peptide gradients will guide the development of translationally relevant constructs designed to facilitate endogenous SC infiltration into defects for nerve regeneration.

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Published In

Biomaterials

DOI

EISSN

1878-5905

ISSN

0142-9612

Publication Date

October 2019

Volume

218

Start / End Page

119335

Related Subject Headings

  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • Schwann Cells
  • Rats, Sprague-Dawley
  • Photoelectron Spectroscopy
  • Peptides
  • Laminin
  • Female
  • Chemotaxis
  • Cell Movement
  • Cell Adhesion
 

Citation

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Motta, C. M. M., Endres, K. J., Wesdemiotis, C., Willits, R. K., & Becker, M. L. (2019). Enhancing Schwann cell migration using concentration gradients of laminin-derived peptides. Biomaterials, 218, 119335. https://doi.org/10.1016/j.biomaterials.2019.119335
Motta, Cecilia M. M., Kevin J. Endres, Chrys Wesdemiotis, Rebecca K. Willits, and Matthew L. Becker. “Enhancing Schwann cell migration using concentration gradients of laminin-derived peptides.Biomaterials 218 (October 2019): 119335. https://doi.org/10.1016/j.biomaterials.2019.119335.
Motta CMM, Endres KJ, Wesdemiotis C, Willits RK, Becker ML. Enhancing Schwann cell migration using concentration gradients of laminin-derived peptides. Biomaterials. 2019 Oct;218:119335.
Motta, Cecilia M. M., et al. “Enhancing Schwann cell migration using concentration gradients of laminin-derived peptides.Biomaterials, vol. 218, Oct. 2019, p. 119335. Epmc, doi:10.1016/j.biomaterials.2019.119335.
Motta CMM, Endres KJ, Wesdemiotis C, Willits RK, Becker ML. Enhancing Schwann cell migration using concentration gradients of laminin-derived peptides. Biomaterials. 2019 Oct;218:119335.
Journal cover image

Published In

Biomaterials

DOI

EISSN

1878-5905

ISSN

0142-9612

Publication Date

October 2019

Volume

218

Start / End Page

119335

Related Subject Headings

  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • Schwann Cells
  • Rats, Sprague-Dawley
  • Photoelectron Spectroscopy
  • Peptides
  • Laminin
  • Female
  • Chemotaxis
  • Cell Movement
  • Cell Adhesion