Direct Evaluation of Pseudomonas aeruginosa Biofilm Mediators in a Chronic Infection Model

Published

Journal Article

ABSTRACTBiofilms contribute toPseudomonas aeruginosapersistence in a variety of diseases, including cystic fibrosis, burn wounds, and chronic suppurative otitis media. However, few studies have directly addressedP. aeruginosabiofilmsin vivo. We used a chinchilla model of otitis media, which has previously been used to study persistentStreptococcus pneumoniaeandHaemophilus influenzaeinfections, to show that structures formedin vivoare biofilms of bacterial and host origin within a matrix that includes Psl, aP. aeruginosabiofilm polysaccharide. We evaluated three biofilm and/or virulence mediators ofP. aeruginosaknown to affect biofilm formationin vitroand pathogenesisin vivo—bis-(3′,5′)-cyclic dimeric GMP (c-di-GMP), flagella, and quorum sensing—in a chinchilla model. We show that c-di-GMP overproduction has a positive impact on bacterial persistence, while quorum sensing increases virulence. We found no difference in persistence attributed to flagella. We conclude from these studies that a chinchilla otitis media model provides a means to evaluate pathogenic mediators ofP. aeruginosaand thatin vitrophenotypes should be examined in multiple infection systems to fully understand their role in disease.

Full Text

Duke Authors

Cited Authors

  • Byrd, MS; Pang, B; Hong, W; Waligora, EA; Juneau, RA; Armbruster, CE; Weimer, KED; Murrah, K; Mann, EE; Lu, H; Sprinkle, A; Parsek, MR; Kock, ND; Wozniak, DJ; Swords, WE

Published Date

  • August 2011

Published In

Volume / Issue

  • 79 / 8

Start / End Page

  • 3087 - 3095

Published By

Electronic International Standard Serial Number (EISSN)

  • 1098-5522

International Standard Serial Number (ISSN)

  • 0019-9567

Digital Object Identifier (DOI)

  • 10.1128/iai.00057-11

Language

  • en