Age-related changes in B cell metabolism.

Published

Journal Article

Antibody responses to vaccinations or infections decline upon aging. In this study we tested if metabolic changes in B cells may contribute to attenuation of responses to influenza vaccination in aged humans. Our data show that aging affects mitochondrial functions in B cells leading to increases in mitochondrial reactive oxygen species (MROS) and mitochondrial mass (MM) in some aged B cell subsets and decreases in expression levels of Sirtuin 1 (SIRT1), Forkhead box protein (FOX)O1 and carnitine palmitoyltransferase 1 (CPT-1). Seahorse analyses showed minor defects in glycolysis in the aged B cells after activation but a strong reduction in oxidative phosphorylation. The analyses of the transcriptome revealed further pronounced defects in one-carbon metabolism, a pathway that is essential for amino acid and nucleotide metabolism. Overall our data support the notion that the declining ability of aged B cells to increase their metabolism following activation contributes to the weakened antibody responses of the elderly.

Full Text

Duke Authors

Cited Authors

  • Kurupati, RK; Haut, LH; Schmader, KE; Ertl, HC

Published Date

  • July 8, 2019

Published In

Volume / Issue

  • 11 / 13

Start / End Page

  • 4367 - 4381

PubMed ID

  • 31283526

Pubmed Central ID

  • 31283526

Electronic International Standard Serial Number (EISSN)

  • 1945-4589

Digital Object Identifier (DOI)

  • 10.18632/aging.102058

Language

  • eng

Conference Location

  • United States