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Cross-linking of porcine acellular dermal matrices negatively affects induced neovessel formation using platelet-rich plasma in a rat model of hernia repair.

Publication ,  Journal Article
Fernandez-Moure, JS; Van Eps, JL; Peterson, LE; Shirkey, BA; Menn, ZK; Cabrera, FJ; Karim, A; Tasciotti, E; Weiner, BK; Ellsworth, WA
Published in: Wound Repair Regen
January 2017

The degree of cross-linking within acellular dermal matrices (ADM) seems to correlate to neovascularization when used in ventral hernia repair (VHR). Platelet-rich plasma (PRP) enhances wound healing through several mechanisms including neovascularization, but research regarding its effect on soft tissue healing in VHR is lacking. We sought to study the effect of cross-linking on PRP-induced neovascularization in a rodent model of bridging VHR. We hypothesized that ADM cross-linking would negatively affect PRP-induced neovessel formation. PRP was extracted and characterized from pooled whole blood. Porcine cross-linked (cADM) and non-cross-linked ADMs (ncADM) were implanted in a rat model of chronic VHR after treatment with saline (control) or PRP. Neovascularization of samples at 2, 4, and 6 weeks was assessed by hematoxylin and eosin and immunohistochemical staining of CD 31. Adhesion severity at necropsy was compared using a previously validated scale. Addition of PRP increased neovascularization in both cADM and ncADM at 2- and 4-week time points but appeared to do so in a dependent fashion, with significantly greater neovascularization in the PRP-treated ncADMs compared to cADMs. Omental adhesions were increased in all PRP-treated groups. Results indicate that, for 2-week measurements when compared with the cADM group without PRP therapy, the mean change in neovascularization due to ncADM was 3.27 (Z = 2.75, p = 0.006), PRP was 17.56 (Z = 14.77, p < 0.001), and the combined effect of ncADM and PRP was 9.41 (Z = 5.6, p < 0.001). The 4-week data indicate that the average neovascularization change due to ncADM was 0.676 (Z = 0.7, p = 0.484), PRP was 7.69 (Z = 7.95, p < 0.001), and combined effect of ncADM and PRP was 5.28 (Z = 3.86, p < 0.001). These findings validate PRP as a clinical adjunct to enhance the native tissue response to implantable biomaterials and suggest that ncADM is more amenable than cADM to induced neovascularization. PRP use could be advantageous in patients undergoing VHR where poor incorporation is anticipated and early-enhanced neovascularization is desired.

Duke Scholars

Published In

Wound Repair Regen

DOI

EISSN

1524-475X

Publication Date

January 2017

Volume

25

Issue

1

Start / End Page

98 / 108

Location

United States

Related Subject Headings

  • Wound Healing
  • Swine
  • Rats
  • Platelet-Rich Plasma
  • Neovascularization, Physiologic
  • Herniorrhaphy
  • Hernia, Ventral
  • Dermatology & Venereal Diseases
  • Biocompatible Materials
  • Animals
 

Citation

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Fernandez-Moure, J. S., Van Eps, J. L., Peterson, L. E., Shirkey, B. A., Menn, Z. K., Cabrera, F. J., … Ellsworth, W. A. (2017). Cross-linking of porcine acellular dermal matrices negatively affects induced neovessel formation using platelet-rich plasma in a rat model of hernia repair. Wound Repair Regen, 25(1), 98–108. https://doi.org/10.1111/wrr.12508
Fernandez-Moure, Joseph S., Jeffrey L. Van Eps, Leif E. Peterson, Beverly A. Shirkey, Zachary K. Menn, Fernando J. Cabrera, Azim Karim, Ennio Tasciotti, Bradley K. Weiner, and Warren A. Ellsworth. “Cross-linking of porcine acellular dermal matrices negatively affects induced neovessel formation using platelet-rich plasma in a rat model of hernia repair.Wound Repair Regen 25, no. 1 (January 2017): 98–108. https://doi.org/10.1111/wrr.12508.
Fernandez-Moure JS, Van Eps JL, Peterson LE, Shirkey BA, Menn ZK, Cabrera FJ, et al. Cross-linking of porcine acellular dermal matrices negatively affects induced neovessel formation using platelet-rich plasma in a rat model of hernia repair. Wound Repair Regen. 2017 Jan;25(1):98–108.
Fernandez-Moure, Joseph S., et al. “Cross-linking of porcine acellular dermal matrices negatively affects induced neovessel formation using platelet-rich plasma in a rat model of hernia repair.Wound Repair Regen, vol. 25, no. 1, Jan. 2017, pp. 98–108. Pubmed, doi:10.1111/wrr.12508.
Fernandez-Moure JS, Van Eps JL, Peterson LE, Shirkey BA, Menn ZK, Cabrera FJ, Karim A, Tasciotti E, Weiner BK, Ellsworth WA. Cross-linking of porcine acellular dermal matrices negatively affects induced neovessel formation using platelet-rich plasma in a rat model of hernia repair. Wound Repair Regen. 2017 Jan;25(1):98–108.
Journal cover image

Published In

Wound Repair Regen

DOI

EISSN

1524-475X

Publication Date

January 2017

Volume

25

Issue

1

Start / End Page

98 / 108

Location

United States

Related Subject Headings

  • Wound Healing
  • Swine
  • Rats
  • Platelet-Rich Plasma
  • Neovascularization, Physiologic
  • Herniorrhaphy
  • Hernia, Ventral
  • Dermatology & Venereal Diseases
  • Biocompatible Materials
  • Animals