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A biomimetic multicellular model of the airways using primary human cells.

Publication ,  Journal Article
Sellgren, KL; Butala, EJ; Gilmour, BP; Randell, SH; Grego, S
Published in: Lab on a chip
September 2014

Microfluidic cell cultures enable investigation of complex physiological tissue properties and functionalities. For convenience, they are often implemented with immortalized cell lines, but primary cells more closely approximate the in vivo biology. Our aim was to develop a biomimetic microfluidic model of the human airway using all primary cells. The model is comprised of airway epithelial cells cultured at an air-liquid interface, lung fibroblasts and polarized microvascular endothelial cells, respectively positioned in three vertically stacked, individually accessible compartments separated by nanoporous membranes. We report device fabrication, a gravity fed microfluidic system, and culture medium able to support functional co-cultures of all three primary human cell types. As characterized by imaging and permeability measurements, airway epithelial cells in microfluidic devices displayed mucociliary differentiation and barrier function. Subjacent fibroblasts and microvascular endothelial cells were added under conditions enabling co-culture for at least 5 days. Microfluidic airway models based on primary human cells in a relevant biomimetic configuration will improve physiological relevance and will enable novel disease modeling and drug development studies.

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Published In

Lab on a chip

DOI

EISSN

1473-0189

ISSN

1473-0197

Publication Date

September 2014

Volume

14

Issue

17

Start / End Page

3349 / 3358

Related Subject Headings

  • Trachea
  • Models, Biological
  • Microfluidics
  • Humans
  • Culture Media
  • Coculture Techniques
  • Biomimetics
  • Analytical Chemistry
  • 40 Engineering
  • 34 Chemical sciences
 

Citation

APA
Chicago
ICMJE
MLA
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Sellgren, K. L., Butala, E. J., Gilmour, B. P., Randell, S. H., & Grego, S. (2014). A biomimetic multicellular model of the airways using primary human cells. Lab on a Chip, 14(17), 3349–3358. https://doi.org/10.1039/c4lc00552j
Sellgren, Katelyn L., Elizabeth J. Butala, Brian P. Gilmour, Scott H. Randell, and Sonia Grego. “A biomimetic multicellular model of the airways using primary human cells.Lab on a Chip 14, no. 17 (September 2014): 3349–58. https://doi.org/10.1039/c4lc00552j.
Sellgren KL, Butala EJ, Gilmour BP, Randell SH, Grego S. A biomimetic multicellular model of the airways using primary human cells. Lab on a chip. 2014 Sep;14(17):3349–58.
Sellgren, Katelyn L., et al. “A biomimetic multicellular model of the airways using primary human cells.Lab on a Chip, vol. 14, no. 17, Sept. 2014, pp. 3349–58. Epmc, doi:10.1039/c4lc00552j.
Sellgren KL, Butala EJ, Gilmour BP, Randell SH, Grego S. A biomimetic multicellular model of the airways using primary human cells. Lab on a chip. 2014 Sep;14(17):3349–3358.
Journal cover image

Published In

Lab on a chip

DOI

EISSN

1473-0189

ISSN

1473-0197

Publication Date

September 2014

Volume

14

Issue

17

Start / End Page

3349 / 3358

Related Subject Headings

  • Trachea
  • Models, Biological
  • Microfluidics
  • Humans
  • Culture Media
  • Coculture Techniques
  • Biomimetics
  • Analytical Chemistry
  • 40 Engineering
  • 34 Chemical sciences