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Fast serotonin voltammetry as a versatile tool for mapping dynamic tissue architecture: I. Responses at carbon fibers describe local tissue physiology.

Publication ,  Journal Article
Abdalla, A; West, A; Jin, Y; Saylor, RA; Qiang, B; Peña, E; Linden, DJ; Nijhout, HF; Reed, MC; Best, J; Hashemi, P
Published in: Journal of neurochemistry
April 2020

It is important to monitor serotonin neurochemistry in the context of brain disorders. Specifically, a better understanding of biophysical alterations and associated biochemical functionality within subregions of the brain will enable better of understanding of diseases such as depression. Fast voltammetric tools at carbon fiber microelectrodes provide an opportunity to make direct evoked and ambient serotonin measurements in vivo in mice. In this study, we characterize novel stimulation and measurement circuitries for serotonin analyses in brain regions relevant to psychiatric disease. Evoked and ambient serotonin in these brain areas, the CA2 region of the hippocampus and the medial prefrontal cortex, are compared to ambient and evoked serotonin in the substantia nigra pars reticulata, an area well established previously for serotonin measurements with fast voltammetry. Stimulation of a common axonal location evoked serotonin in all three brain regions. Differences are observed in the serotonin release and reuptake profiles between these three brain areas which we hypothesize to arise from tissue physiology heterogeneity around the carbon fiber microelectrodes. We validate this hypothesis mathematically and via confocal imaging. We thereby show that fast voltammetric methods can provide accurate information about local physiology and highlight implications for chemical mapping. Cover Image for this issue: doi: 10.1111/jnc.14739.

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Published In

Journal of neurochemistry

DOI

EISSN

1471-4159

ISSN

0022-3042

Publication Date

April 2020

Volume

153

Issue

1

Start / End Page

33 / 50

Related Subject Headings

  • Substantia Nigra
  • Serotonin
  • Prefrontal Cortex
  • Neurology & Neurosurgery
  • Models, Theoretical
  • Microelectrodes
  • Mice, Inbred C57BL
  • Mice
  • Mental Disorders
  • Medial Forebrain Bundle
 

Citation

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Abdalla, A., West, A., Jin, Y., Saylor, R. A., Qiang, B., Peña, E., … Hashemi, P. (2020). Fast serotonin voltammetry as a versatile tool for mapping dynamic tissue architecture: I. Responses at carbon fibers describe local tissue physiology. Journal of Neurochemistry, 153(1), 33–50. https://doi.org/10.1111/jnc.14854
Abdalla, Aya, Alyssa West, Yunju Jin, Rachel A. Saylor, Beidi Qiang, Edsel Peña, David J. Linden, et al. “Fast serotonin voltammetry as a versatile tool for mapping dynamic tissue architecture: I. Responses at carbon fibers describe local tissue physiology.Journal of Neurochemistry 153, no. 1 (April 2020): 33–50. https://doi.org/10.1111/jnc.14854.
Abdalla A, West A, Jin Y, Saylor RA, Qiang B, Peña E, et al. Fast serotonin voltammetry as a versatile tool for mapping dynamic tissue architecture: I. Responses at carbon fibers describe local tissue physiology. Journal of neurochemistry. 2020 Apr;153(1):33–50.
Abdalla, Aya, et al. “Fast serotonin voltammetry as a versatile tool for mapping dynamic tissue architecture: I. Responses at carbon fibers describe local tissue physiology.Journal of Neurochemistry, vol. 153, no. 1, Apr. 2020, pp. 33–50. Epmc, doi:10.1111/jnc.14854.
Abdalla A, West A, Jin Y, Saylor RA, Qiang B, Peña E, Linden DJ, Nijhout HF, Reed MC, Best J, Hashemi P. Fast serotonin voltammetry as a versatile tool for mapping dynamic tissue architecture: I. Responses at carbon fibers describe local tissue physiology. Journal of neurochemistry. 2020 Apr;153(1):33–50.
Journal cover image

Published In

Journal of neurochemistry

DOI

EISSN

1471-4159

ISSN

0022-3042

Publication Date

April 2020

Volume

153

Issue

1

Start / End Page

33 / 50

Related Subject Headings

  • Substantia Nigra
  • Serotonin
  • Prefrontal Cortex
  • Neurology & Neurosurgery
  • Models, Theoretical
  • Microelectrodes
  • Mice, Inbred C57BL
  • Mice
  • Mental Disorders
  • Medial Forebrain Bundle