The Effect of Ex Situ Perfusion in a Swine Limb Vascularized Composite Tissue Allograft on Survival up to 24 Hours.
Journal Article (Journal Article)
Purpose
To test the potential for the ex situ limb perfusion system to prolong limb allograft survival up to 24 hours.Methods
We used 20 swine for the study. In group 1 (control), 4 limbs were perfused with heparin solution and preserved at 4°C for 6 hours. In group 2, 4 limbs were perfused with autologous blood at 27°C to 32°C for 24 hours. In both groups, limbs were transplanted orthotopically to recipients and monitored for 12 hours. In addition to perfusion parameters, we recorded perfusate gases and electrolytes (pH, pCO2, pO2, O2 saturation, Na, K, Cl, Ca, HCO3, glucose, and lactate) and obtained functional electrostimulation hourly throughout the experiment. Histology samples were obtained for TUNEL staining and single-muscle fiber contractility testing.Results
In both groups, hemodynamic variables of circulation remained stable throughout the experiment. Neuromuscular electrical stimulation remained intact until the end of reperfusion in group 2 vs no response in group 1. In group 2, a gradual increase in lactate levels during pump perfusion returned to normal after transplantation. Compared with the contralateral limb in group 2, single-muscle fiber contractility testing showed no significant difference at the end of the experiment.Conclusions
We demonstrated extended limb survival up to 24 hours using normothermic pulsatile perfusion and autologous blood.Clinical relevance
Successful prolongation of limb survival using ex situ perfusion methods provides with more time for revascularization of an extremity.Full Text
Cited Authors
- Ozer, K; Rojas-Pena, A; Mendias, CL; Bryner, BS; Toomasian, C; Bartlett, RH
Published Date
- January 2016
Published In
Volume / Issue
- 41 / 1
Start / End Page
- 3 - 12
PubMed ID
- 26710728
Electronic International Standard Serial Number (EISSN)
- 1531-6564
International Standard Serial Number (ISSN)
- 0363-5023
Digital Object Identifier (DOI)
- 10.1016/j.jhsa.2015.11.003
Language
- eng