An optically transparent membrane supports shear stress studies in a three-dimensional microfluidic neurovascular unit model.

Journal Article (Journal Article)

We report a microfluidic blood-brain barrier model that enables both physiological shear stress and optical transparency throughout the device. Brain endothelial cells grown in an optically transparent membrane-integrated microfluidic device were able to withstand physiological fluid shear stress using a hydrophilized polytetrafluoroethylene nanoporous membrane instead of the more commonly used polyester membrane. A functional three-dimensional microfluidic co-culture model of the neurovascular unit is presented that incorporates astrocytes in a 3D hydrogel and enables physiological shear stress on the membrane-supported endothelial cell layer.

Full Text

Duke Authors

Cited Authors

  • Sellgren, KL; Hawkins, BT; Grego, S

Published Date

  • November 12, 2015

Published In

Volume / Issue

  • 9 / 6

Start / End Page

  • 061102 -

PubMed ID

  • 26594261

Pubmed Central ID

  • PMC4644144

Electronic International Standard Serial Number (EISSN)

  • 1932-1058

International Standard Serial Number (ISSN)

  • 1932-1058

Digital Object Identifier (DOI)

  • 10.1063/1.4935594


  • eng