Molecular biology techniques and their applicability to the study of diabetes and hypertension: the renin-angiotensin system as an example.

Published

Journal Article (Review)

Recently developed molecular biology techniques lend themselves to the study of both normal physiology and pathophysiology. This review discusses a number of such techniques and their applicability to the study of diabetes and hypertension with the renin-angiotensin system as an example. The demonstration of specific mRNA in tissue provides strong evidence that the protein for which it encodes is expressed there. The contribution of several methods to demonstrate mRNA expression including Northern analysis, slot blot analysis, solution hybridization, in situ hybridization, run-on assays, and polymerase chain reaction are discussed in the light of how these are used to study renin-angiotensin system mechanisms. Additionally, recent developments in the study of gene transfer and identification of genes are reviewed.

Full Text

Duke Authors

Cited Authors

  • Ingelfinger, JR; Jung, F; Tang, SS

Published Date

  • October 1992

Published In

Volume / Issue

  • 3 / 4 Suppl

Start / End Page

  • S18 - S26

PubMed ID

  • 1281007

Pubmed Central ID

  • 1281007

International Standard Serial Number (ISSN)

  • 1046-6673

Language

  • eng

Conference Location

  • United States