Directed differentiation and neurite extension of mouse embryonic stem cell on aligned poly(lactide) nanofibers functionalized with YIGSR peptide.

Published

Journal Article

End-functional PLLA nanofibers were fabricated into mats of random or aligned fibers and functionalized post-spinning using metal-free "click chemistry" with the peptide Tyr-Ile-Gly-Ser-Arg (YIGSR). Fibers that were both aligned and functionalized with YIGSR were found to significantly increase the fraction of mouse embryonic stem cells (mESC) expressing neuron-specific class III beta-tubulin (TUJ1), the level of neurite extension and gene expression for neural markers compared to mESC cultured on random fiber mats and unfunctionalized matrices. Precise functionalization of degradable polymers with bioactive peptides created translationally-relevant materials that capitalize on the advantages of both synthetic and natural systems, while mitigating the classic limitations of each.

Full Text

Duke Authors

Cited Authors

  • Callahan, LAS; Xie, S; Barker, IA; Zheng, J; Reneker, DH; Dove, AP; Becker, ML

Published Date

  • December 2013

Published In

Volume / Issue

  • 34 / 36

Start / End Page

  • 9089 - 9095

PubMed ID

  • 24008044

Pubmed Central ID

  • 24008044

Electronic International Standard Serial Number (EISSN)

  • 1878-5905

International Standard Serial Number (ISSN)

  • 0142-9612

Digital Object Identifier (DOI)

  • 10.1016/j.biomaterials.2013.08.028

Language

  • eng