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Maximizing phenotype constraint and extracellular matrix production in primary human chondrocytes using arginine-glycine-aspartate concentration gradient hydrogels.

Publication ,  Journal Article
Smith Callahan, LA; Childers, EP; Bernard, SL; Weiner, SD; Becker, ML
Published in: Acta biomaterialia
July 2013

New systematic approaches are necessary to determine and optimize the chemical and mechanical scaffold properties for hyaline cartilage generation using the limited cell numbers obtained from primary human sources. Peptide functionalized hydrogels possessing continuous variations in physico-chemical properties are an efficient three-dimensional platform for studying several properties simultaneously. Herein, we describe a polyethylene glycol dimethacrylate (PEGDM) hydrogel system possessing a gradient of arginine-glycine-aspartic acid peptide (RGD) concentrations from 0mM to 10mM. The system is used to correlate primary human osteoarthritic chondrocyte proliferation, phenotype maintenance and extracellular matrix (ECM) production to the gradient hydrogel properties. Cell number and chondrogenic phenotype (CD14:CD90 ratios) were found to decline in regions with higher RGD concentrations, while regions with lower RGD concentrations maintained cell number and phenotype. Over three weeks of culture, hydrogel regions containing lower RGD concentrations experience an increase in ECM content compared to regions with higher RGD concentrations. Variations in actin amounts and vinculin organization were observed within the RGD concentration gradients that contribute to the differences in chondrogenic phenotype maintenance and ECM expression.

Duke Scholars

Published In

Acta biomaterialia

DOI

EISSN

1878-7568

ISSN

1742-7061

Publication Date

July 2013

Volume

9

Issue

7

Start / End Page

7420 / 7428

Related Subject Headings

  • Oligopeptides
  • Materials Testing
  • Hydrogels
  • Humans
  • Extracellular Matrix
  • Dose-Response Relationship, Drug
  • Chondrocytes
  • Cells, Cultured
  • Biomedical Engineering
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Smith Callahan, L. A., Childers, E. P., Bernard, S. L., Weiner, S. D., & Becker, M. L. (2013). Maximizing phenotype constraint and extracellular matrix production in primary human chondrocytes using arginine-glycine-aspartate concentration gradient hydrogels. Acta Biomaterialia, 9(7), 7420–7428. https://doi.org/10.1016/j.actbio.2013.04.005
Smith Callahan, Laura A., Erin P. Childers, Sharon L. Bernard, Scott D. Weiner, and Matthew L. Becker. “Maximizing phenotype constraint and extracellular matrix production in primary human chondrocytes using arginine-glycine-aspartate concentration gradient hydrogels.Acta Biomaterialia 9, no. 7 (July 2013): 7420–28. https://doi.org/10.1016/j.actbio.2013.04.005.
Smith Callahan LA, Childers EP, Bernard SL, Weiner SD, Becker ML. Maximizing phenotype constraint and extracellular matrix production in primary human chondrocytes using arginine-glycine-aspartate concentration gradient hydrogels. Acta biomaterialia. 2013 Jul;9(7):7420–8.
Smith Callahan, Laura A., et al. “Maximizing phenotype constraint and extracellular matrix production in primary human chondrocytes using arginine-glycine-aspartate concentration gradient hydrogels.Acta Biomaterialia, vol. 9, no. 7, July 2013, pp. 7420–28. Epmc, doi:10.1016/j.actbio.2013.04.005.
Smith Callahan LA, Childers EP, Bernard SL, Weiner SD, Becker ML. Maximizing phenotype constraint and extracellular matrix production in primary human chondrocytes using arginine-glycine-aspartate concentration gradient hydrogels. Acta biomaterialia. 2013 Jul;9(7):7420–7428.
Journal cover image

Published In

Acta biomaterialia

DOI

EISSN

1878-7568

ISSN

1742-7061

Publication Date

July 2013

Volume

9

Issue

7

Start / End Page

7420 / 7428

Related Subject Headings

  • Oligopeptides
  • Materials Testing
  • Hydrogels
  • Humans
  • Extracellular Matrix
  • Dose-Response Relationship, Drug
  • Chondrocytes
  • Cells, Cultured
  • Biomedical Engineering