Facile fabrication of "dual click" one- and two-dimensional orthogonal peptide concentration gradients.
Published
Journal Article
Peptides, proteins, and extracellular matrix act synergistically to influence cellular function at the biotic-synthetic interface. However, identifying the individual and cooperative contributions of the various combinations and concentration regimes is extremely difficult. The confined channel deposition method we describe affords highly tunable orthogonal reactive concentration gradients that greatly expand the dynamic range, spatial control, and chemical versatility of the reactive silanes that can be controllably deposited. Using metal-free "dual click" immobilization chemistries, multiple peptides with a variety of functionality can be immobilized efficiently and reproducibly enabling optimal concentration profiling and the assessment of synergistic interactions.
Full Text
Duke Authors
Cited Authors
- Ma, Y; Zheng, J; Amond, EF; Stafford, CM; Becker, ML
Published Date
- March 2013
Published In
Volume / Issue
- 14 / 3
Start / End Page
- 665 - 671
PubMed ID
- 23330789
Pubmed Central ID
- 23330789
Electronic International Standard Serial Number (EISSN)
- 1526-4602
International Standard Serial Number (ISSN)
- 1525-7797
Digital Object Identifier (DOI)
- 10.1021/bm301731h
Language
- eng