Facile fabrication of "dual click" one- and two-dimensional orthogonal peptide concentration gradients.

Published

Journal Article

Peptides, proteins, and extracellular matrix act synergistically to influence cellular function at the biotic-synthetic interface. However, identifying the individual and cooperative contributions of the various combinations and concentration regimes is extremely difficult. The confined channel deposition method we describe affords highly tunable orthogonal reactive concentration gradients that greatly expand the dynamic range, spatial control, and chemical versatility of the reactive silanes that can be controllably deposited. Using metal-free "dual click" immobilization chemistries, multiple peptides with a variety of functionality can be immobilized efficiently and reproducibly enabling optimal concentration profiling and the assessment of synergistic interactions.

Full Text

Duke Authors

Cited Authors

  • Ma, Y; Zheng, J; Amond, EF; Stafford, CM; Becker, ML

Published Date

  • March 2013

Published In

Volume / Issue

  • 14 / 3

Start / End Page

  • 665 - 671

PubMed ID

  • 23330789

Pubmed Central ID

  • 23330789

Electronic International Standard Serial Number (EISSN)

  • 1526-4602

International Standard Serial Number (ISSN)

  • 1525-7797

Digital Object Identifier (DOI)

  • 10.1021/bm301731h

Language

  • eng