Universal gradient substrates for "click" biofunctionalization


Journal Article

A novel and versatile method for fabricating continuously variable concentration gradients of surface-conjugated biomolecules is reported. This technology utilizes graded UV oxidation of self-assembled monolayers (SAM) as well as converts the resulting carboxylate gradient into an increasing density of alkyne functionalities appropriate for click-chemistry surface conjugation of biomolecules. This approach to the fabrication of bioactive surface gradients has been to develop and characterize a functional gradient substrate to which a variety of species can be attached. In the final fabrication step of the Universal Gradient Substrate, a bifunctional propargyl-derivatized linker was attached to the acid gradient by using standard amidation methods to yield a surface possessing an increasing concentration of alkyne groups. cell function. This technology for fabricating continuously variable density gradients of bioactive molecules has a wide range of potential uses in biomaterials.

Full Text

Duke Authors

Cited Authors

  • Gallant, ND; Lavery, KA; Amis, EJ; Becker, ML

Published Date

  • April 6, 2007

Published In

Volume / Issue

  • 19 / 7

Start / End Page

  • 965 - 969

International Standard Serial Number (ISSN)

  • 0935-9648

Digital Object Identifier (DOI)

  • 10.1002/adma.200602221

Citation Source

  • Scopus