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Interaction of gold nanoparticles with common human blood proteins.

Publication ,  Journal Article
Lacerda, SHDP; Park, JJ; Meuse, C; Pristinski, D; Becker, ML; Karim, A; Douglas, JF
Published in: ACS nano
January 2010

In order to better understand the physical basis of the biological activity of nanoparticles (NPs) in nanomedicine applications and under conditions of environmental exposure, we performed an array of photophysical measurements to quantify the interaction of model gold NPs having a wide range of NP diameters with common blood proteins. In particular, absorbance, fluorescence quenching, circular dichroism, dynamic light scattering, and electron microscopy measurements were performed on surface-functionalized water-soluble gold NPs having a diameter range from 5 to 100 nm in the presence of common human blood proteins: albumin, fibrinogen, gamma-globulin, histone, and insulin. We find that the gold NPs strongly associate with these essential blood proteins where the binding constant, K, as well as the degree of cooperativity of particle--protein binding (Hill constant, n), depends on particle size and the native protein structure. We also find tentative evidence that the model proteins undergo conformational change upon association with the NPs and that the thickness of the adsorbed protein layer (bare NP diameter <50 nm) progressively increases with NP size, effects that have potential general importance for understanding NP aggregation in biological media and the interaction of NP with biological materials broadly.

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Published In

ACS nano

DOI

EISSN

1936-086X

ISSN

1936-0851

Publication Date

January 2010

Volume

4

Issue

1

Start / End Page

365 / 379

Related Subject Headings

  • Spectrometry, Fluorescence
  • Scattering, Radiation
  • Protein Binding
  • Particle Size
  • Nanoscience & Nanotechnology
  • Microscopy, Electron
  • Metal Nanoparticles
  • Light
  • Humans
  • Gold
 

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Lacerda, S. H. D. P., Park, J. J., Meuse, C., Pristinski, D., Becker, M. L., Karim, A., & Douglas, J. F. (2010). Interaction of gold nanoparticles with common human blood proteins. ACS Nano, 4(1), 365–379. https://doi.org/10.1021/nn9011187
Lacerda, Silvia H De Paoli, Jung Jin Park, Curt Meuse, Denis Pristinski, Matthew L. Becker, Alamgir Karim, and Jack F. Douglas. “Interaction of gold nanoparticles with common human blood proteins.ACS Nano 4, no. 1 (January 2010): 365–79. https://doi.org/10.1021/nn9011187.
Lacerda SHDP, Park JJ, Meuse C, Pristinski D, Becker ML, Karim A, et al. Interaction of gold nanoparticles with common human blood proteins. ACS nano. 2010 Jan;4(1):365–79.
Lacerda, Silvia H. De Paoli, et al. “Interaction of gold nanoparticles with common human blood proteins.ACS Nano, vol. 4, no. 1, Jan. 2010, pp. 365–79. Epmc, doi:10.1021/nn9011187.
Lacerda SHDP, Park JJ, Meuse C, Pristinski D, Becker ML, Karim A, Douglas JF. Interaction of gold nanoparticles with common human blood proteins. ACS nano. 2010 Jan;4(1):365–379.
Journal cover image

Published In

ACS nano

DOI

EISSN

1936-086X

ISSN

1936-0851

Publication Date

January 2010

Volume

4

Issue

1

Start / End Page

365 / 379

Related Subject Headings

  • Spectrometry, Fluorescence
  • Scattering, Radiation
  • Protein Binding
  • Particle Size
  • Nanoscience & Nanotechnology
  • Microscopy, Electron
  • Metal Nanoparticles
  • Light
  • Humans
  • Gold