Improving the Quality and Reproducibility of Flow Cytometry in the Lung. An Official American Thoracic Society Workshop Report.

Journal Article (Journal Article)

Defining responses of the structural and immune cells in biologic systems is critically important to understanding disease states and responses to injury. This requires accurate and sensitive methods to define cell types in organ systems. The principal method to delineate the cell populations involved in these processes is flow cytometry. Although researchers increasingly use flow cytometry, technical challenges can affect its accuracy and reproducibility, thus significantly limiting scientific advancements. This challenge is particularly critical to lung immunology, as the lung is readily accessible and therefore used in preclinical and clinical studies to define potential therapeutics. Given the importance of flow cytometry in pulmonary research, the American Thoracic Society convened a working group to highlight issues and technical challenges to the performance of high-quality pulmonary flow cytometry, with a goal of improving its quality and reproducibility.

Full Text

Duke Authors

Cited Authors

  • Tighe, RM; Redente, EF; Yu, Y-R; Herold, S; Sperling, AI; Curtis, JL; Duggan, R; Swaminathan, S; Nakano, H; Zacharias, WJ; Janssen, WJ; Freeman, CM; Brinkman, RR; Singer, BD; Jakubzick, CV; Misharin, AV

Published Date

  • August 2019

Published In

Volume / Issue

  • 61 / 2

Start / End Page

  • 150 - 161

PubMed ID

  • 31368812

Pubmed Central ID

  • PMC6670040

Electronic International Standard Serial Number (EISSN)

  • 1535-4989

Digital Object Identifier (DOI)

  • 10.1165/rcmb.2019-0191ST


  • eng

Conference Location

  • United States