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TiO2 nanoparticles generate superoxide and alter gene expression in human lung cells.

Publication ,  Journal Article
Jayaram, DT; Kumar, A; Kippner, LE; Ho, P-Y; Kemp, ML; Fan, Y; Payne, CK
Published in: RSC advances
January 2019

TiO2 nanoparticles are widely used in consumer products and industrial applications, yet little is understood regarding how the inhalation of these nanoparticles impacts long-term health. This is especially important for the occupational safety of workers who process these materials. We used RNA sequencing to probe changes in gene expression and fluorescence microscopy to image intracellular reactive oxygen species (ROS) in human lung cells incubated with low, non-cytotoxic, concentrations of TiO2 nanoparticles. Experiments were designed to measure changes in gene expression following an acute exposure to TiO2 nanoparticles and changes inherited by progeny cells. We observe that TiO2 nanoparticles lead to significant (>2000 differentially expressed genes) changes in gene expression following a 24 hour incubation. Following this acute exposure, the response dissipates with only 34 differentially expressed genes in progeny cells. The progeny cells adapt to this initial exposure, observed when re-challenged with a second acute TiO2 nanoparticle exposure. Accompanying these changes in gene expression is the production of intracellular ROS, specifically superoxide, along with changes in oxidative stress-related genes. These experiments suggest that TiO2 nanoparticles adapt to oxidative stress through transcriptional changes over multiple generations of cells.

Duke Scholars

Published In

RSC advances

DOI

EISSN

2046-2069

ISSN

2046-2069

Publication Date

January 2019

Volume

9

Issue

43

Start / End Page

25039 / 25047

Related Subject Headings

  • 34 Chemical sciences
  • 03 Chemical Sciences
 

Citation

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Jayaram, D. T., Kumar, A., Kippner, L. E., Ho, P.-Y., Kemp, M. L., Fan, Y., & Payne, C. K. (2019). TiO2 nanoparticles generate superoxide and alter gene expression in human lung cells. RSC Advances, 9(43), 25039–25047. https://doi.org/10.1039/c9ra04037d
Jayaram, Dhanya T., Ashwath Kumar, Linda E. Kippner, Po-Yi Ho, Melissa L. Kemp, Yuhong Fan, and Christine K. Payne. “TiO2 nanoparticles generate superoxide and alter gene expression in human lung cells.RSC Advances 9, no. 43 (January 2019): 25039–47. https://doi.org/10.1039/c9ra04037d.
Jayaram DT, Kumar A, Kippner LE, Ho P-Y, Kemp ML, Fan Y, et al. TiO2 nanoparticles generate superoxide and alter gene expression in human lung cells. RSC advances. 2019 Jan;9(43):25039–47.
Jayaram, Dhanya T., et al. “TiO2 nanoparticles generate superoxide and alter gene expression in human lung cells.RSC Advances, vol. 9, no. 43, Jan. 2019, pp. 25039–47. Epmc, doi:10.1039/c9ra04037d.
Jayaram DT, Kumar A, Kippner LE, Ho P-Y, Kemp ML, Fan Y, Payne CK. TiO2 nanoparticles generate superoxide and alter gene expression in human lung cells. RSC advances. 2019 Jan;9(43):25039–25047.
Journal cover image

Published In

RSC advances

DOI

EISSN

2046-2069

ISSN

2046-2069

Publication Date

January 2019

Volume

9

Issue

43

Start / End Page

25039 / 25047

Related Subject Headings

  • 34 Chemical sciences
  • 03 Chemical Sciences