Evaluation of attenuated tumor antigens and the implications for peptide-based cancer vaccine development

Journal Article (Journal Article)

INTRODUCTION: Peptide vaccines offer anti-tumor efficacy with very low toxicity. However, repeat stimulation with an immunogenic peptide leads to activation induced cell death (AICD), decreasing efficacy. We engineered variants of an immunogenic peptide (E39) and tested their ability to induce a robust, sustainable immune response. METHODS: Multiple variants of E39 were created by exchanging 1 or 2 amino acids. We tested the PBMC proliferation, cytokine production and cytolytic activity induced by each variant peptide. RESULTS: Repeated stimulation with E39 likely led to in vitro AICD, while stimulation with E39' led to T-cell proliferation with less evidence of AICD, modest cytokine production and high CTL activity. CONCLUSIONS: E39' appears to be the optimal variant of E39 for inducing effective long-term immunity.

Full Text

Duke Authors

Cited Authors

  • Berry, JS; Vreeland, TJ; Hale, DF; Jackson, DO; Trappey, AF; Greene, JM; Hardin, MO; Herbert, GS; Clifton, GT; Peoples, GE

Published Date

  • January 1, 2017

Published In

Volume / Issue

  • 8 / 7

Start / End Page

  • 1255 - 1262

Electronic International Standard Serial Number (EISSN)

  • 1837-9664

Digital Object Identifier (DOI)

  • 10.7150/jca.16450

Citation Source

  • Scopus