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A Transcriptional Lineage of the Early C. elegans Embryo.

Publication ,  Journal Article
Tintori, SC; Osborne Nishimura, E; Golden, P; Lieb, JD; Goldstein, B
Published in: Developmental cell
August 2016

During embryonic development, cells must establish fates, morphologies, and behaviors in coordination with one another to form a functional body. A prevalent hypothesis for how this coordination is achieved is that each cell's fate and behavior is determined by a defined mixture of RNAs. Only recently has it become possible to measure the full suite of transcripts in a single cell. Here we quantify genome-wide mRNA abundance in each cell of the Caenorhabditis elegans embryo up to the 16-cell stage. We describe spatially dynamic expression, quantify cell-specific differential activation of the zygotic genome, and identify genes that were previously unappreciated as being critical for development. We present an interactive data visualization tool that allows broad access to our dataset. This genome-wide single-cell map of mRNA abundance, alongside the well-studied life history and fate of each cell, describes at a cellular resolution the mRNA landscape that guides development.

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Published In

Developmental cell

DOI

EISSN

1878-1551

ISSN

1534-5807

Publication Date

August 2016

Volume

38

Issue

4

Start / End Page

430 / 444

Related Subject Headings

  • Zygote
  • Transcriptome
  • Sequence Analysis, RNA
  • RNA, Messenger
  • Gene Expression Regulation, Developmental
  • Gene Expression Profiling
  • Embryonic Development
  • Embryo, Nonmammalian
  • Developmental Biology
  • Cell Lineage
 

Citation

APA
Chicago
ICMJE
MLA
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Tintori, S. C., Osborne Nishimura, E., Golden, P., Lieb, J. D., & Goldstein, B. (2016). A Transcriptional Lineage of the Early C. elegans Embryo. Developmental Cell, 38(4), 430–444. https://doi.org/10.1016/j.devcel.2016.07.025
Tintori, Sophia C., Erin Osborne Nishimura, Patrick Golden, Jason D. Lieb, and Bob Goldstein. “A Transcriptional Lineage of the Early C. elegans Embryo.Developmental Cell 38, no. 4 (August 2016): 430–44. https://doi.org/10.1016/j.devcel.2016.07.025.
Tintori SC, Osborne Nishimura E, Golden P, Lieb JD, Goldstein B. A Transcriptional Lineage of the Early C. elegans Embryo. Developmental cell. 2016 Aug;38(4):430–44.
Tintori, Sophia C., et al. “A Transcriptional Lineage of the Early C. elegans Embryo.Developmental Cell, vol. 38, no. 4, Aug. 2016, pp. 430–44. Epmc, doi:10.1016/j.devcel.2016.07.025.
Tintori SC, Osborne Nishimura E, Golden P, Lieb JD, Goldstein B. A Transcriptional Lineage of the Early C. elegans Embryo. Developmental cell. 2016 Aug;38(4):430–444.
Journal cover image

Published In

Developmental cell

DOI

EISSN

1878-1551

ISSN

1534-5807

Publication Date

August 2016

Volume

38

Issue

4

Start / End Page

430 / 444

Related Subject Headings

  • Zygote
  • Transcriptome
  • Sequence Analysis, RNA
  • RNA, Messenger
  • Gene Expression Regulation, Developmental
  • Gene Expression Profiling
  • Embryonic Development
  • Embryo, Nonmammalian
  • Developmental Biology
  • Cell Lineage