Cell-Based Therapy Restores Olfactory Function in an Inducible Model of Hyposmia.

Journal Article (Journal Article)

Stem cell-based therapies have been proposed as a strategy to replace damaged tissues, especially in the nervous system. A primary sensory modality, olfaction, is impaired in 12% of the US population, but lacks treatment options. We report here the development of a novel mouse model of inducible hyposmia and demonstrate that purified tissue-specific stem cells delivered intranasally engraft to produce olfactory neurons, achieving recovery of function. Adult mice were rendered hyposmic by conditional deletion of the ciliopathy-related IFT88 gene in the olfactory sensory neuron lineage and following experimentally induced olfactory injury, received either vehicle or stem cell infusion intranasally. Engraftment-derived olfactory neurons were identified histologically, and functional improvements were measured via electrophysiology and behavioral assay. We further explored mechanisms in culture that promote expansion of engraftment-competent adult olfactory basal progenitor cells. These findings provide a basis for translational research on propagating adult tissue-specific sensory progenitor cells and testing their therapeutic potential.

Full Text

Duke Authors

Cited Authors

  • Kurtenbach, S; Goss, GM; Goncalves, S; Choi, R; Hare, JM; Chaudhari, N; Goldstein, BJ

Published Date

  • June 11, 2019

Published In

Volume / Issue

  • 12 / 6

Start / End Page

  • 1354 - 1365

PubMed ID

  • 31155504

Pubmed Central ID

  • 31155504

Electronic International Standard Serial Number (EISSN)

  • 2213-6711

Digital Object Identifier (DOI)

  • 10.1016/j.stemcr.2019.05.001

Language

  • eng

Conference Location

  • United States