Histologic changes of mesenchymal stem cell repair of tympanic membrane perforation.

Journal Article (Journal Article;Review)

CONCLUSION: Mesenchymal stem-cells are good candidates for cell-therapy of chronic tympanic membranes perforations. OBJECTIVES: To determine the effects of cell-based therapy in tympanic membrane perforations. METHODS: Young C57BL/6 mice were anesthetized with intraperitoneal administration of ketamine and xylazine and randomly divided into three groups (n = 4 ears/group) that underwent bilateral sub-total pars tensa perforations of equal sizes using a sterile 27-gauge needle under a surgical microscope. Six-to-eight hours after injury, one group of mice did not receive treatment (acute perforation control), and the last two groups were treated with BM-MSCs embedded within HA scaffolds previously soaked in PBS to rinse culture media residues to avoid confounders and were euthanized 1 or 2 weeks after treatment. RESULTS: Untreated tympanic membrane perforations developed a hyper-cellular infiltrate surrounding the injury site, while BM-MSC treated eardrums showed a reduced inflammatory response after the first week and a restoration of the trilaminar configuration 2 weeks after treatment, mimicking a normal tympanic membrane.

Full Text

Duke Authors

Cited Authors

  • Goncalves, S; Bas, E; Langston, M; Grobman, A; Goldstein, BJ; Angeli, S

Published Date

  • April 2017

Published In

Volume / Issue

  • 137 / 4

Start / End Page

  • 411 - 416

PubMed ID

  • 27960618

Electronic International Standard Serial Number (EISSN)

  • 1651-2251

Digital Object Identifier (DOI)

  • 10.1080/00016489.2016.1261411


  • eng

Conference Location

  • England